Editing Elisa protocol (section)

=== <br/>Reagents ===

<br/>1. Coating BufferAnhydrous Na2CO 3 , 1.5 g<br/>Anhydrous NaHCO3, 2.93 g<br/>Distilled water, 1 liter, pH to 9.6

For an alternative coating buffer use ELISA coating buffer (BUF030)2. Blocking bufferPhosphate Buffered Saline (PBS) containing 1% w/v BSA

For an alternative blocking buffer, use either ELISA BSA Block (BUF032), ELISA Ultrablock (BUF033), or ELISA Synblock (BUF034)3. Wash bufferPhosphate Buffered Saline containing 0.05% v/v Tween®-20

For an alternative wash buffer use ELISA wash buffer (BUF031)Recommended Substrates and Stop SolutionsTMB Core+ (BUF062), for use with HRP-conjugated antibodies. Stop with 2M H2SO4.<br/>pNPP (BUF044), for use with alkaline phosphatase-conjugated antibodies. Stop with 1M NaOH.