Editing PAAV-Mcs (section)

== '''Result intepretation/Summary'''  ==
<pre>sequencing using CMV-F
NNNNNNNNNNNNNNNNNNNCNGANCTCGTTTAGTGNCCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATAGAAGACACCGGGACCGATCCAGCCTCCGCGGATTCGAATCCCGGCCGGGAACGGTGCATTGGAACGCGGATTCCCCGTGCCAAGAGTGACGTAAGTACCGCCTATAGAGTCTATAGGCCCACAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGT
TTATCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCAT
TCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATATAAATTGT
AACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAA
GGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTCCCACAGCTTCTGGG
CAACGTGCTGGTCTGTGTGCTGCCCCNNNANTTTGNCTAAAAATTGGCATTCGAACTCCCATTGAANACCCNGTGNGTNC
NNTNNACCTGACCNCCTTTCTNNNNCNNNAAACAGGACGCTATGTCATAGCTGACGCTGTAGGTATATCANTTCNTTGTC
TNNTTTAGCTNTTAGCNANGNTNTTTCCCACNAANACCNCNNN


 Score = 1040 bits (563),  Expect = 0.0
 Identities = 590/605 (98%), Gaps = 5/605 (1%)
 Strand=Plus/Plus

Query  25    CTCGTTTAGTG-NCCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATA  83
             |||||||||||  |||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  726   CTCGTTTAGTGAACCGTCAGATCGCCTGGAGACGCCATCCACGCTGTTTTGACCTCCATA  785

Query  84    GAAGACACCGGGACCGATCCAGCCTCCGCGGATTCGAATCCCGGCCGGGAACGGTGCATT  143
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  786   GAAGACACCGGGACCGATCCAGCCTCCGCGGATTCGAATCCCGGCCGGGAACGGTGCATT  845

Query  144   GGAACGCGGATTCCCCGTGCCAAGAGTGACGTAAGTACCGCCTATAGAGTCTATAGGCCC  203
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  846   GGAACGCGGATTCCCCGTGCCAAGAGTGACGTAAGTACCGCCTATAGAGTCTATAGGCCC  905

Query  204   ACAAAAAATGctttcttcttttaatatacttttttgtttatcttatttctaatactttcc  263
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  906   ACAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATACTTTCC  965

Query  264   ctaatctctttctttcAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCT  323
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  966   CTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCT  1025

Query  324   AAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATAT  383
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  1026  AAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATAT  1085

Query  384   TTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATC  443
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  1086  TTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATC  1145

Query  444   CAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAG  503
             ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  1146  CAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAG  1205

Query  504   CTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTCCCACAGCTTCTGGGCAA  563
             ||||||||||||||||||||||||||||||||||||||||||||||||||| ||||||||
Sbjct  1206  CTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTCCCACAGCTCCTGGGCAA  1265

Query  564   CGTGCTGGTCTGTGTGCTGCCCCNNNANTTTGNCTAAA-AATTGGCATTCGAAC-TCCCA  621
             ||||||||||||||||||| |||   | |||| | ||| |||||| |||||||| ||  |
Sbjct  1266  CGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGC-AAAGAATTGGGATTCGAACATCG-A  1323

Query  622   TTGAA  626
             |||||
Sbjct  1324  TTGAA  1328
</pre> 
<pre>
Sequence using hGH-PA-R CCAGCTTGGTTCCCAATAGA

good
254_pAAV-MCS-hGH-PA-R_A12.ab1
NNNNNNNNNNNNNNNNNNNCNNNNNNNNTTGCNCNTTGCTCCNTACCACCCCCCTCCACCCCATAATATTATAGAAGGAC
ACCTAGTCAGACAAAATGATGCAACTTAATTTTATTAGGACAAGGCTGGTGGGCACTGGAGTGGCAACTTCCAGGGCCAG
GAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGTAGATCTCTCGAGAAGCTTGGTACCCTGCAGGTCGACTCTAGA
GGATCCACCGGTGAATTCAATCGATGTTCGAATCCCAATTCTTTGCCAAAGTGATGGGCCAGCACACAGACCAGCACGTT
GCCCAGGAGCTGTGGGAGGAAGATAAGAGGTATGAACATGATTAGCAAAAGGGCCTAGCTTGGACTCAGAATAATCCAGC
CTTATCCCAACCATAAAATAAAAGCAGAATGGTAGCTGGATTGTAGCTGCTATTAGCAATATGAAACCTCTTACATCAGT
TACAATTTATATGCAGAAATATTTATATGCAGAAATATTGCTATTGCCTTAACCCAGAAATTATCACTGTTATTCTTTAG
AATGGTGCAAAGAGGCATGATACATTGTATCATTATTGCCCTGAAAGAAAGAGATTAGGGAAAGTATTAGAAATAAGATA
AACAAAAAAGTATATTAAAAGAAGAAAGCATTTTTTGTGGGCCTATAGACTCTATAGGCGGTACTTACGTCNNNCTTGGC
NCGGGGAAATCCGCGTTTCCNATGNNNCGTTCCCGNNNNNNNATTCNAA


rev-comp
TTNGAATNNNNNNNCGGGAACGNNNCATNGGAAACGCGGATTTCCCCGNGCCAAGNNNGACGTAAGTACCGCCTATAGAGTCTATAGGCCCACAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCTTATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATGCCTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATATTTCTGCATATAAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGCTAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGGATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTCCCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTGGGATTCGAACATCGATT
EcoR1 AgeI    BamH1  XbaI     Sal1   Pst1  HindIII    KpnI    Xho1  BglII
GAATTC ACCGGT GGATCC TCTAGA GTCGAC CTGCAG   GGTACC   AAGCTT   CTCGAG AGATCT


ACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTANGGAGCAANGNGCAANNNNNNNNGNNNNNNNNNNNNNNNNNNN


blast vs pAAV-MCS



 Score = 1290 bits (698),  Expect = 0.0
 Identities = 714/727 (98%), Gaps = 2/727 (0%)
 Strand=Plus/Plus


Query  15   CGGGAACGNNNCATNGGAAACGCGGATTTCCCCGNGCCAAGNNNGACGTAAGTACCGCCT  74
            ||||||||   ||| || |||||||| ||||||| ||||||   ||||||||||||||||
Sbjct  12   CGGGAACGGTGCATTGG-AACGCGGA-TTCCCCGTGCCAAGAGTGACGTAAGTACCGCCT  69


Query  75   ATAGAGTCTATAGGCCCACAAAAAATGctttcttcttttaatatacttttttgtttatct  134
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  70   ATAGAGTCTATAGGCCCACAAAAAATGCTTTCTTCTTTTAATATACTTTTTTGTTTATCT  129


Query  135  tatttctaatactttccctaatctctttctttcAGGGCAATAATGATACAATGTATCATG  194
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  130  TATTTCTAATACTTTCCCTAATCTCTTTCTTTCAGGGCAATAATGATACAATGTATCATG  189


Query  195  CCTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATA  254
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  190  CCTCTTTGCACCATTCTAAAGAATAACAGTGATAATTTCTGGGTTAAGGCAATAGCAATA  249


Query  255  TTTCTGCATATAAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGC  314
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  250  TTTCTGCATATAAATATTTCTGCATATAAATTGTAACTGATGTAAGAGGTTTCATATTGC  309


Query  315  TAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGG  374
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  310  TAATAGCAGCTACAATCCAGCTACCATTCTGCTTTTATTTTATGGTTGGGATAAGGCTGG  369


Query  375  ATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTC  434
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  370  ATTATTCTGAGTCCAAGCTAGGCCCTTTTGCTAATCATGTTCATACCTCTTATCTTCCTC  429


Query  435  CCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTG  494
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  430  CCACAGCTCCTGGGCAACGTGCTGGTCTGTGTGCTGGCCCATCACTTTGGCAAAGAATTG  489


Query  495  GGATTCGAACATCGATTGAATTCACCGGTGGATCCTCTAGAGTCGACCTGCAGGGTACCA  554
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  490  GGATTCGAACATCGATTGAATTCACCGGTGGATCCTCTAGAGTCGACCTGCAGGGTACCA  549


Query  555  AGCTTCTCGAGAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGC  614
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Objct  550  AGCTTCTCGAGAGATCTACGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGC  609


Query  615  CCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCAT  674
            ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Sbjct  610  CCTGGAAGTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCAT  669


Query  675  TTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTANGGAGCAA  734
            |||||||||||||||||||||||||||||||||||||||||||||||||||| |||||||
Sbjct  670  TTTGTCTGACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGTATGGAGCAA  729


Query  735  NGNGCaa  741
             | ||||
Sbjct  730  GGGGCAA  736

</pre> 
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