Editing Plasmid MaxiPrep by PEG Q XL (section)

== '''Optional protocol:'''<br>  ==

1. Column: RESOURCE Q, 6 ml (prepacked with SOURCE 15 Q)<br>Sample: from step 16, dilute to 0.4 M NaCl (2.5 folds)<br>Sample load: 1 to 140 ml<br>Sample conductivity: 38 mS/cm<br>Buffer A: 25 mM Tris-HCl, 1 mm EDTA, pH 8.0<br>Buffer B&nbsp;: 25 mM Tris-HCl, 1 mM EDTA, 1.0 M NaCl, pH 8.0<br>Flow velocity: 300 cm/hr<br>Gradient: 40 -100%B, 20 CV<br>Then precipitate DNA as step 17 to 21.<br> 

<br> 

2, Column: Plasmid Select, FineLINE 70,<br>14 cm bed height (CV = 566 ml)<br>Buffer A: 2 M (NH4)2SO4, 10 mM EDTA, 100 mM Tris-HCI, pH 7.0 <br> 

Buffer B: 1.4 M NaCl, 2 M (NH4)2SO4, 10 mM EDTA, 100 mM Tris-HCI, pH 7.0<br>Flow velocity: 120 cm/h<br> 

<br> 

3. SOURCE 30Q, FineLINE 35, 12 cm bed height (CV = 137 ml)<br>Buffer A: 0.4M NaCl, 10 mM EDTA, 100 mM Tris-HCI, pH 7.0 <br> 

Buffer B: 1.0M NaCl, 10 mM EDTA, 100 mM Tris-HCI, pH 7.0<br>Flow velocity: 120 cm/h<br><br><br> 

<br>