Editing Plasmid buffers (section)

=== Buffers for&nbsp;epochbiolabs.com maxiprep columns ===

VP1: 50mM Tris-HCl, pH 8.0, 10mM EDTA, With 100ug/mL RNase A; 10 ml per maxiprep

VP2: 0.2N NaOH, 1% SDS; 10 ml per maxiprep; leave at RT for 5 min to digest RNA<br/>VP3: 3M KoAc, pH 5.5

Shake column with 5 ml 98% ethanol, equilibrate column with 20 ml VPN, load supernatnat from lysate.<br/>VPN: 0.1M NaoAc, pH 5.1, 1.2M NaCl; Wash column with 30 ml VPN

VPE: 0.1M Tris-HCl, pH8.5, 1.35M NaCl, 15% Isopropanol; Elute DNA,&nbsp; 10 ml.

Precipitate DNA using 7.5 ml isopropanol, spin 30 min at 12,000 rpm. Wash pellet with 70% ethanol.