Editing Enzyme Properties (section)

==== DNA Endonucleases ====

{| border="1" cellpadding="5" cellspacing="0"
|-
| Enzyme
| Substrate
| Activity
| Products
| Applications
| Heat Inactivationa
|-
| RNase-Free DNase I<br/>(bovine pancreas)
| dsDNA and ssDNA
| Activated by divalent cations. In presence of Mg2+, it
cleaves each DNA strand randomly and independently, preferentially adjacent to pyrimidines. In presence of Mn2+, it cleaves both strands simultaneously, generating fragments with blunt ends of 1-2-base
overhangs
| Oligos and dNMPs with 5´ phosphate and 3´-OH
| Removing DNA from RNA preps. Random nicking of dsDNA. DNase footprinting
| 20 min, 75°C
|-
| Baseline-ZERO™ DNase
| dsDNA and ssDNA
| Digests dsDNA or ssDNA down to mononucleotides
| Mononucleotides
| Removing DNA from RNA preps
| 20 min, 75°C
|-
| Endonuclease IV,&nbsp;E. coli
| dsDNA with an abasic site
| Cleaves sugar-phosphate bond 5´ of an abasic site
| dsDNA with single-nucleotide gaps. The cleaved ssDNA strand has a 3´-OH
| DNA repair and antitumor drug research. Base Excision Sequence Scanning of DNA containing dUMPs
| NT
|-
| T4 Endonuclease V
| UV-irradiated DNA with thymine dimers
| First cleaves N-glycosidic bond 5´ of thymine dimers, then cleaves sugar-phosphate bond 3´ of the abasic site
| Nicked dsDNA with an abasic site at the 3´ end of the cut and thymine-dimer bases at the 5´ end of the cut
| Research on repair of DNA exposed to UV light
| NT
|-
| Lambda Terminase
| dsDNA with&nbsp;cossites
| Cleaves both strands at bacteriophage lambda&nbsp;cos&nbsp;sites
| 5´ ends with overhangs 12 bases in length
| Rapid sizing or restriction mapping of BAC, fosmid, or cosmid clones
| NT
|}