Editing Nutrient Broth (section)

=== ATCC Protocol ===

ATCC® Number: 6633D-5 ™<br>Deposited As Bacillus subtilis (Ehrenberg) Cohn<br>Strain Designations: Genomic DNA from strain NRS 231 [ATCC 6633] 

ATCC Medium: 415<br>Potato Extract Agar/Broth<br>For Solid Media:<br>Nutrient Agar .......................................23.0 g<br>Potato Extract (see below)......................20.0 ml<br>DI Water..............................................980.0 mL<br>Autoclave at 121 ºC<br>To prepare potato extract, boil 300 g peeled, diced potatoes in 500 ml distilled water for&nbsp;half an hour. Decant the infusion and use in the above medium. (Put through cheese&nbsp;cloth a few times.)<br>For Broth<br>Nutrient Broth....................................8.0 g<br>Potato Extract (see above) .................20.0 mL<br>DI Water.........................................980.0 mL<br>Autoclave at 121 ºC 

Growth Conditions <br>Temperature: 26°C<br>Atmosphere: Aerobic 



'''http://iwi.eldoc.ub.rug.nl/FILES/root/2011/PLoSOneBlom/2011PLoSOneBlom.pdf'''

TY medium (10 g/L trypton, 5 g/L yeast extract, 5 g/L NaCl) in batch mode&nbsp;at 37uC in a 16 L fermentor using a working volume of 12 L.<br>Cultures were aerated at 40 L/min and 400 rpm