Editing Plasmid MaxiPrep using DMAE Column (section)

=== Columns:  ===

To pack a new DMAE column, get two polyethylene frits and a plastic 25ml column. 

Using a pair of tweezers, push one frit all the way to the bottom of the column. Then pipette 6ml Fractogel DMAE on top. Slowly draw a vacuum from the bottom until the liquid level reaches near the top of the settled resin. (If the vacuum is too strong, the resin will get packed too tightly and gravity drip will therefore be too slow. For comfort, you can place the column on a compatible vacuum manifold, e.g. the blue Promega Wizard manifold.) Push the second frit down carefully so it rests flat at the top of the resin and does not squeeze the resin too much. Add some equilibration buffer and draw the vacuum through slowly until there are no more air pockets visible in the resin. The column should now be wetted all the way through and thus allow a modest rate of gravity drip. From this point on, do not let columns dry out. Columns left overnight at any step will usually stay moist.