Editing Yijia LI 4 2 2018 (section)

== Date:4/6/2018 ==

'''939 suclone'''

'''Size:780bp'''

'''Insert:939pJet1.2'''

'''Vector:1727 pT7A30'''

'''Enzymes:Nde1+Xho1'''

[[File:939subclone.JPG|RTENOTITLE]]

'''938 subclone'''

'''Size:1145bp'''

'''Insert:938pJet1.2'''

'''Vector:1727 pT7A30'''

'''Enzymes:Nde1+Xho1'''

[[File:938subclone.JPG|RTENOTITLE]]

'''3122 subclone'''

'''Size:1743bp'''

'''Insert:3122pJet1.2'''

'''Vector:1727 pT7A30'''

'''Enzymes:Nde1+Xho1'''

[[File:3122subclone.JPG|RTENOTITLE]]

'''3127 subclone'''

'''Size:408 bp'''

'''Insert:939pJet1.2'''

'''Vector:1727 pT7A30'''

'''Enzymes:Nde1+HindIII'''

[[File:3127subclone.JPG|RTENOTITLE]]

'''986 PCR'''

[[File:986pcr.JPG|RTENOTITLE]]

'''986 digestion as vector'''

'''Enzymes:HindIII+Xho1'''

[[File:986vectordigestion.JPG|RTENOTITLE]]

'''945 vector diegstion'''

'''Enzymes:Xba1+Nco1'''

[[File:945vectordigestion.JPG|RTENOTITLE]]

'''3166 PCR'''

'''Primer:3166-F+3166-R'''

'''Template:3017 pCEH0'''

'''Extension time:1min 30s'''

'''Size:1234bp'''

[[File:Pcr3166.JPG|RTENOTITLE]]

'''3152 subclone'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| &nbsp;Lane#
| Name
| Note
|-
| 1
| Undigested vector
| - control
|-
| 2
| digested vector
| - control
|-
| 3
| digested vector---3016 pCEH0
| for subclone
|-
| 4
| digested insert---3009pMiniT2
| for subclone
|}

[[File:3152subclone.JPG|RTENOTITLE]]

'''3129 digestion as vector'''

'''Enzymes:Sal1+BsrG1'''

[[File:3129vector diegstoinfor31653166.JPG|RTENOTITLE]]

'''945 subclone'''

'''Size:1326 bp'''

'''Enzymes:Xba1+Nco1'''

[[File:945subclone.JPG|RTENOTITLE]]

'''3165 PCR'''

[[File:PCRof3165.JPG|RTENOTITLE]]

'''944 PCR'''

'''Size:2334bp. might be wrong, because two bands in the gel.'''

[[File:PCRof944.JPG|RTENOTITLE]]

'''931 subclone'''

'''Vector:1518 pET28b digested with Nde1+Xho1'''

'''F1:1174 bp, digested with Nde1+HindIII'''

'''F2:986 bp, digested with HindIII+ Xho1'''

[[File:931subclone.JPG|RTENOTITLE]]

'''985 subclone'''

'''Vector:1987 pT7A3 digested with''''''&nbsp;Nde1-Xho1'' '''Insert:983(973-2 pMiniT2) digested with Nde1+Sal1'''

'''Size:500 bp'''

[[File:985subclone.JPG|RTENOTITLE]]
[[Category:My ELN]]