Editing Adenovirus Purification using Q Membrane (section)

== Conclusions  ==
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Adenoviral vectors are widely used as highly efficient gene 

transfer vehicles in a variety of biological research strategies including human gene therapy. However, issues regarding purification processes still present a challenge. In that regard, any method that renders the purification process that is faster, cheaper and safer than what is currently available, may represent a major advance in adenoviral technology. In the present work, we report the development of a chromatographic process incorporating the new Mustang Q membrane as an alternative to CsCl gradient purification. Mustang Q membrane yields high recoveries of infectious viral particles and processes rAd particles from lysate in a fraction of the time required using the traditional CsCl method. One separation can be performed in less than 30 minutes compared to three days. In addition, Pall Mustang Q Acrodisc filter units are ideal devices to purify viral particles for a range for any lab-scale process and are scalable in both disposable or reusable formats for large-scale purification requirements. Overall, this novel purification method proved to be faster and more cost effective than the CsCl gradient purification. 
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[[Category:Protocols]]