Editing Phage Display from Synthetic Library (section)

=== Materials ===

{| border="1"
|-
| '''Name'''
| '''Company'''
| '''Catalog Number'''
| '''Comments'''
|-
| MATERIALS
|-
| New Brunswick Innova44 stackable incubator shaker
| Eppendorf
| M1282-0004
|-
| Liquidator 96 channel manual benchtop pipetting system
| Anachem Ltd
| LIQ-96-200
|-
| Microplate shaker
| VWR
| 12620-926
|-
| ThermoScientific Sorvall ST-40 benchtop centrifuge
| Thermo Product
| 75004525
|-
| ELx405 Select Deep Well Microplate Washer
| Biotek
| ELX405USD
|-
| Custom High Throughput Automated Phage Selection Robot
| S&P Robotics
|-
| Plastic conical Falcon tube: 50 ml
| VWR
| 21008-178
|-
| Plastic conical Falcon tube: 15 ml
| VWR
| 89039-668
|-
| Axygen 1.7 ml microcentrifuge tubes
| Corning
| MCT-175-L-C
|-
| 96-well/384-well Maxisorp plate
| Sigma
| M9410-1CS
|-
| Corning 96-well V-bottom deep-well block
| Corning
| 3960
|-
| Axygen Mini-tube 96-well sterile microplate blue box
| Corning
| AXY-MTS-06-C-R-S
|-
| Breathable adhesive plate sealing film
| VWR
| 60941-084
|-
| REAGENTS
|-
| Name
| Company
| Catalog Number
|-
| polyethylene glycol
| Bioshop
| PEG800.1
|-
| yeast extract
| Bioshop
| YEX401.1
|-
| bio-tryptone
| Bioshop
| TRP402.1
|-
| N-Z amine
| Sigma
| C7290
|-
| glucose
| Sigma
| G8270-1KG
|-
| lactose
| Bioshop
| LAC234
|-
| glycerol
| Bioshop
| GLY001.500
|-
| carbenicillin
| Bioshop
| CAR544.10
|-
| kanamycin
| Bioshop
| KAN201.25
|-
| tetracycline
| Bioshop
| TET701.25
|-
| Tween 20
| Bioshop
| TWN510.500
|-
| monobasic potassium phophate
| Bioshop
| PPM302.1
|-
| dibasic sodium phosphate
| Anachemia
| 84486-440
|-
| sodium chloride
| Bioshop
| SOD001.10
|-
| potassium chloride
| Bioshop
| POC308.1
|-
| calcium chloride
| Bioshop
| CCL302.500
|-
| magnesium sulphate
| EMD
| MX0070-1
|-
| magnesium chloride
| Bioshop
| MAG510.500
|-
| NH<sub>4</sub>Cl
| Amresco
| 0621-1KG
|-
| Na<sub>2</sub>SO<sub>4</sub>
| Bioshop
| SOS513.500
|-
| agar
| Bioshop
| AGR001.500
|-
| SybrSafe DNA gel stain
| Invitrogen
| S33102
|-
| phosphoric acid
| Acros Organics
| 201140010
|-
| lysozyme
| Bioshop
| LYS702.25
|-
| benzonase
| Novagen
| 71205
|-
| Triton X-100
| Bioshop
| TRX506.500
|-
| protease inhibitor cocktail tablets
| Roche
| 11 836 170 001
|-
| Ni-NTA resin
| Qiagen
| 1018240
|-
| 1,000x helper phage (M13K07) stock (10<sup>13</sup> phage per ml)
| NEB
| N0315S
|-
| HRP conjugated M13-specific antibody (anti-M13-HRP).
| GE Healthcare
| 27-9241-01
|-
| TMB substrate: mix equal volumes of TMB and H<sub>2</sub>O<sub>2</sub> peroxidase substrate
| KPL
| 50-76-00
|-
| dNTPs
| Biobasic
| DD0056
|-
| Taq polymerase
| Genscript
| E00007
|-
| Exonuclease
| GE Healthcare
| EZ0073X-EZ
|-
| Shrimp alkaline phosphatase
| GE Healthcare
| E70092Z-EZ
|}
[[Category:Antibody]][[Category:Antibodies]]