Editing Plasmid MaxiPrep by PEG Q XL (section)

== '''Optional method to further reduce endotoxin level'''<br>  ==

Dissolve DNA in water and add Triton X-114 to a final concentration of 0.1-1%.<br> 

Incubate the solution on ice, mix occasionally, for 30 min.<br> 

Heat the solution to 42 oC for 5 min.<br> 

Spin at 20,000× g for 5 min or filter. <br> 

Add 0.7 volumes of isopropanol to supernatant 

Spin for 10 to 15 min to precipitate DNA. 

Wash DNA with 80% ethanol 

Dry the resulting pellet in a biosafety hood and resuspend in TE. <br> 

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