Editing Protein Expression 293 (section)

=== Materials ===

{|
|-
| &lt;tbody&gt;
|-
| '''Name'''
| '''Company'''
| '''Catalog Number'''
| '''Comments'''
|-
| Alkaline phosphatase (BCIP/NBT) liquid substrate solution
| Sigma
| B6404
|-
| Antibiotic/Antimycotic, 100X
| Invitrogen
| 15240062
|-
| Anti-HA affinity matrix, clone 3F10
| Roche
| 1815016
|-
| Anti-HA murine mAb, clone 16B12
| Covance
| MMS-101P
|-
| Cell culture flask, T75 cm<sup>2</sup> tissue culture treated
| Corning
| 430641
|-
| Cell culture flask, T225 cm<sup>2</sup> tissue culture treated
| Corning
| 431082
|-
| Cell culture plates,6-well tissue culture treated
| Corning
| 3516
|-
| Cell factory, 10-layer CellSTACK
| Corning
| 3312
|-
| Centramate Omega 5K Cassette
| Pall
| OS005C12
|-
| Centramate Omega 30K Cassette
| Pall
| OS030C12
|-
| Chromatography glass column, 1.0x10 cm
| Kontes
| 4204001010
|-
| Ciprofloxacin
| Sigma
| 17850
|-
| CO<sub>2</sub>
|-
| Dulbecco's modified Eagle's media (DMEM)
| Sigma
| D5796
|-
| Fetal bovine serum (FBS), heat inactivated
| Invitrogen
| 12484-028
|-
| FuGENE HD transfection reagent
| Promega
| 4709691001
|-
| GeneJuice transfection reagent
| EMD/Merck
| 70967-6
|-
| Glycine
| Sigma
| G8898
|-
| Goat anti-mouse IgG F(ab')<sub>2</sub> alkaline
| Thermo Scientific
| 31324
|-
| phosphatase-conjugated antibody
|-
| Hemagglutinin (HA) peptide, 100 mg
| Genscript
| custom synthesis
|-
| (sequence: YPYDVPDYA; 95% purity)
|-
| HEK 293T cells
| ATCC
| CRL-11268
|-
| Household bleach (4% w/v sodium hypochlorite)
| various brands are available
|-
| Immobilon-P PVDF membrane
| Millipore
| IPVH07850
|-
| MiniPrep plasmid purification kit, PureLink Quick
| Invitrogen
| K2100-11
|-
| MaxiPrep plasmid purification kit, PureLink HiPure
| Invitrogen
| K2100-07
|-
| NaN<sub>3</sub>
| Sigma
| S8032
|-
| pDISPLAY expression vector
| Invitrogen
| V660-20
|-
| Penicillin/streptomycin (pen/strep), 100X
| Invitrogen
| 15140-122
|-
| Phosphate-buffered saline (PBS), sterile 1X
| Sigma
| D8537
|-
| Polyethyleneimine (PEI), linear 25 kDa
| Polyscience
| 23966
|-
| Skim milk dry powder
| Carnation
|-
| Stericup-GP PES vacuum filtration unit,
| Millipore
| SCGPU05RE
|-
| 0.22 μm, 500 ml capacity
|-
| Trypan blue
| Invitrogen
| 15250061
|-
| Trypsin-EDTA, 0.05% (w/v)
| Invitrogen
| 25300-054
|-
| Tween-20
| Sigma
| P7949
|-
| Valproic acid
| Sigma
| P4543
|-
| Centramate tangential flow system
| Pall
|-
| CO<sub>2</sub> humidified incubator, standard 6.0 cu. ft.
| various brands are available
|-
| Electrophoresis and transfer unit
| various brands are available
|-
| Incubator, 37 °C
| various brands are available
|-
| &lt;/tbody&gt;
|}

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