Editing QPCR mix (section)

=== 2X SYBR Mix ===

This recipe is to make 1ml of '''2X qPCR master mix''' using Taq DNA polymerase (with thermopol buffer) available from New England Biolabs. This mix is enough to make an entire 96 well plate of 20μL qPCR reactions. After this mix is prepared it should be kept on ice until use.

:
*705μL Water
:*200μL Thermopol Buffer (with Mg<sup>2+</sup>)
:*25μL dNTP mix (10mM each)
:*40μL ROX dye (see note)
:*10μL Taq DNA Polymerase (~15 units)
:*5μL each primer (100μM)
:*10μL SYBR Green II (100x)

==== Notes ====

*ROX is supplied in a 25μM stock solution. The 40μL listed above is the amount used for Applied Biosystems 7000, 7300, 7700, 7900HT, StepOne, and PRISM models. If using an AB 7500 only use 1/10 the amount of ROX suggested here.
*If not using ROX (or using less ROX) substitute the remaining volume with water.