Editing Plasmid MaxiPrep HIC (section)

==== Purify DNA using Butyl 650M ====

[[File:Plasmid HIC 9 9 2012.jpg|RTENOTITLE]]

1. Plasmid purified with silica miniprep spin column, using P1 with RNase, and N3

2. Plasmid purified using 650M, add CaCl2 (1 M).

3. Plasmid purified from FT of #2, using silica miniprep spin column

4. Plasmid purified using 650M, add CaCl2 (1 M), did not add NaCl at step 13.

5. Plasmid purified from FT of #4 using silica miniprep spin column.

6. Plasmid purified using 650M, add 60 ml 3M ammonium sulfate at step 5.

7. Plasmid purified with silica miniprep spin column, with P1 without RNase, and P3.

Load 10 ul.

{| width="526" cellspacing="1" cellpadding="1" border="1"
|-
| <br/>
| 1<br/>
| 2<br/>
| 3<br/>
| 4<br/>
| 5<br/>
| 6<br/>
| 7<br/>
|-
| OD260<br/>
| 45.8<br/>
| 64.6<br/>
| 8.9<br/>
| 8.7<br/>
| 3.2<br/>
| 580<br/>
| 157<br/>
|-
| 260/280<br/>
| 1.96<br/>
| 1.85<br/>
| 2.01<br/>
| 1.48<br/>
| -<br/>
| 2.1<br/>
| 2.08<br/>
|}