Editing DSMZ Cells (section)

=== Method 4 ===

Thermotoga maritima DSM 3109, T. neapolitana DSM 4359, and T. elfii DSM 9442 were grown in a modified version of the ATCC 1977 medium (see van Ooteghem et al. 2002) containing 1 g cysteine.HCl l -1 as reducing agent, and glucose, xylose, arabinose or glycerol as carbon substrate. The medium also contained 2 g yeast extract l -1 and 2 g peptone l -1 , which are needed by these bacteria. Potassium phosphate was increased from 0.6 to 6.3 g l -1 to increase the buffering capacity of the growth medium (Eriksen et al. 2008), while the concentrations of MgCl.H 2 0 and CaCl 2 .H 2 0 were reduced to 0.2 and 0.01 g l -1 , respectively, to prevent formation of precipitates. MgCl, CaCl 2 and the carbon source (glucose, xylose, arabinose, or glycerol) were autoclaved in a separate solution before mixed with a solution containing the remaining medium constituents. A few crystals of resazurin were added as redox indicator.