Editing Expression of HA NA using Baculovoirus (section)

=== Harvesting and infecting High Five cells<br>  ===

1. Detach High Five cells from 5 T175 cm2 flasks by tapping the flasks with your hand. Collect the cell suspension and transfer into 50 ml centrifugation tubes.<br> 2. Spin at 1,200 x g for 7 min at room temperature. Remove supernatant by decanting and unite the pellets by resuspending them in 15 ml of P3 stock.<br> 3. Let sit for 15 min in the flow hood (Figure 5).<br> 4. Transfer 200 ml of Hyclone SFX culture medium into a clean, sterile 1,000 ml shaker flask (without buffles, sealed with aluminium foil before autoclaving, should not have been used for bacterial cultures before).<br> 5. Transfer the P3/cell suspension into the shaker flask. Seal with sterile aluminium foil and transfer flask into a shaker. Shake at 70 rpm at 28 °C for 72-96 hr.