Editing Silica Nanoparticles (section)

== Study on the Effect of Synthesis Parameters of SilicaNanoparticles Entrapped with Rifampicin<br>  ==

DOI: 10.3303/ACOS1311044<br> 

Nor Ain Zainal a , Syamsul Rizal Abd Shukor* a , Hajaratul Azwana Ab. Wab b ,Khairunisak Abdul Razak<br> 

<br>The materials required for the synthesis of silica nanoparticles are described as follows. Tween 80 - viscous<br>liquid, trimethoxyvinylsilane (TMVS 98% pure), and rifampicin were obtained from Sigma-Aldrich Co. (MO,<br>USA). 2-butanol (99% pure), and ammonium hydroxide solution 10 M (31.5% NH 3 pure) were obtained from<br>Fischer Scientific (Fairlawn, NJ). All these chemicals were of analytical grade and used without any<br>purification. De-ionized water from a Millipore filtration system (operating at 18.2 MΩ cm) was used in this<br>study.<br>2.2 Preparation of Silica Nanoparticles<br>Silica nanoparticles were prepared by micelles formation approach. First, 5.5 mL Tween 80 was dissolved in<br>200 mL of de-ionized water. The mixture was stirred for 15 minutes before 200 μL of prepared NH 3 ( 1 mL NH 3<br>solution was dissolved in 1 mL de-ionized water) was added to ensure the pH is maintain in the range 9 - 11.<br>Then, 2-butanol was poured into the mixture and continuously stirred for five minutes. The mixture was then<br>transferred into a preheated reactor at the set temperature and continuously stirred at 320 rpm for one hour.<br>After that, the prepared rifampicin drug solution ( 0.0839 g of rifampicin dissolved in 1.5 mL methanol) was<br>added into the above mixture and continuously stirs under the same condition. After an hour,<br>trimethoxyvinylsilane (TMVS) as silica precursor was added. The mixture was left overnight with continuously<br>stirred at 320 rpm and maintain with the set temperature, which yield a total volume of 250 mL. The effect of<br>synthesis temperature, butanol and TMVS on the particle size of silica was investigated. The produced silica<br>nanocolloids were then subjected to dialysis process for five days to remove the excess surfactant. Finally, the<br>sample will then be collected in the bottle and stored in the refrigerator until further test.<br> 

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[[Image:Silica nanoparticle size.png]] 

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