Editing 2328 pL42-Tth-PolyA-Pol

== '''Cloning of 2328 pL42-Tth-PolyA-Pol'''  ==

'''Purpose:''' Cloning of2328 pL42-Tth-PolyA-Pol'''==''' 

'''Date: '''2015-1--- 

'''Notebook:0''' 

'''Page:''' 

'''Clone ID:2328''' 

'''Experimenters:'''&nbsp; shanyi 

== '''Results'''  ==

=== CLONING  ===

PCR from Tth HB27 genomic DNA 

<br> 1 

2328-Tth-PolyA-Pol-Nde1-F atcagcatatggcccaCatggacttcccct 

2328-Tth-PolyA-Pol-R ctgggcctcCaggagggcct 

<br> 2 

2328-Tth-PolyA-Pol-F aggccctcctGgaggcccag 

2328-Tth-PolyA-Pol-Xho1-R TGGTGctcgagatgggggtgatctggggttc 

[[Image:2326 2327 2328pcr.JPG]]<br> 

1+ 2 

2328-Tth-PolyA-Pol-Nde1-F atcagcatatggcccaCatggacttcccct 

2328-Tth-PolyA-Pol-Xho1-R TGGTGctcgagatgggggtgatctggggttc <br> 

[[Image:2328PCR.JPG]]<br> Digest with Nde1 + Xho1<br> 

[[Image:Digest for 2326 2327 2328.JPG]]<br> 

=== Colony Screening  ===

Date: 2014_9<br> 

Make miniprep, use Nde1 + Xho1 to find positive clone<br> 

[[Image:2325 2326 2327 2328digest.JPG]]<br> 

== '''Result intepretation/Summary'''  ==

*<br>

== '''Conclusion'''  ==

[[Category:Cloning]]