Editing 2795

== 2795 gibson ==

[[File:2795 Gibson 1.JPG|RTENOTITLE]]

[[File:2746 2795 gibson.JPG|RTENOTITLE]]

[[File:2795 gibson vector.JPG|RTENOTITLE]]



== 2795 subclone ==

20200617 digest 2795_pJet2.1 with NdeI/NotI. The size should be 2181bp, but sometimes the maker is not that accurate. So we select two bands for further ligation with the vector 2794 digested with&nbsp;NdeI/NotI.

[[File:2746 2773 2779 2795 pJet digestion.JPG|RTENOTITLE]]

20200618 2795 vector comes from 2794 digested with&nbsp;NdeI/NotI. Time course showed the two hours digestion looks best.&nbsp;

[[File:2795 subclone vector NdeI NcoI.JPG|RTENOTITLE]]

20200619 2795-T7-VEE clone PCR was performed. Primers used will produce 2153bp product. So the insert band between 3-5kb looks like the one we want.&nbsp;

[[File:2746 2795 clone PCR.JPG|RTENOTITLE]]

== PCR ==

== 2795 mRNA cleancap ==

[[File:2795 1st PCR.JPG|RTENOTITLE]]

[[File:2795 2nd PCR.JPG|RTENOTITLE]]

[[File:2795 PCR product confirmation.JPG|RTENOTITLE]]
[[Category:Cloning]]