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<pre>not working---> PCR from 2931 Gibson mix cloning 2935-F4 TCTAGTCCGCCAAGTCTAGcatatgCTAGCtagagaataaac 1uM 2935-F1 atcatatgCTAGCtagagaataaactagtattcttctggtccccacagactc 1 uM 2938-F1 cttctggtccccacagactcagagagaaccGCCACCATGGACTATAAGGAC 2939-R1 CTATGTAAGCAGCTTGCGGCCGCTATTCCTTTTTCTTTTTTGCCTG (CAGGCAAAAAAGAAAAAGGAATAGCGGCCGCAAGCTGCTTACATAG) ======== Use Nde1-AflII for cloning into 2895 or 2926 2939-R2 TGTAAGCAGCTTaAGCTATTCCTTTTTCTTTTTTGCCTGG (CCAGGCAAAAAAGAAAAAGGAATAGCTtAAGCTGCTTACA) === Enzyme-free cloning <s>1uM 2935-F1 atcatatgCTAGCtagagaataaactagtattcttctggtccccacagactc</s> <s>EFC-F CatatgaagctTAATACGACTCACTATAagGagaataaactagtattcttc </s> PCR insert from 2931 EFC-F1 CatatgaagctTAATACGACTCACTATAagGagaataaac EFC- F2 TCACTATAagGagaataaac tagtattcttctggtcccca 1 uM 2938-F1 cttctggtccccacagactcagagagaaccGCCACCATGGACTATAAGGAC 2939-EFC-R caCTAGTCCCCATCCTCGAGCTATTCCTTTTTCTTTTTTG (CAAAAAAGAAAAAGGAATAGCTCGAGGATGGGGACTAGtg) PCR from vector 2893 EFC-V-F CTCGAGGATGGGGACTAGtg EFC-V-R (2942-R) TGAGTCGTATTAagcttcatatGAGCTCGCGTTGCTGGCG (CGCCAGCAACGCGAGCTCatatgaagctTAATACGACTCA) digest with DpnI, purify, Gibson mix and transform 10G </pre> [[File:2935 2938 2939 2942.JPG|RTENOTITLE]] T7ag-pA-eCas9 <pre>Nde1 HindIII catatgaagct ( T7 ag) TAATACGACTCACTATA agG agaataaactagtattcttc tggtccccacagactcagagagaacc GCCACCATGGACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCCCCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACGACGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAAGTACAAAGAGATTTTCTTCGACCAGAGCAAGAACGGCTACGCCGGCTACATTGACGGCGGAGCCAGCCAGGAAGAGTTCTACAAGTTCATCAAGCCCATCCTGGAAAAGATGGACGGCACCGAGGAACTGCTCGTGAAGCTGAACAGAGAGGACCTGCTGCGGAAGCAGCGGACCTTCGACAACGGCAGCATCCCCCACCAGATCCACCTGGGAGAGCTGCACGCCATTCTGCGGCGGCAGGAAGATTTTTACCCATTCCTGAAGGACAACCGGGAAAAGATCGAGAAGATCCTGACCTTCCGCATCCCCTACTACGTGGGCCCTCTGGCCAGGGGAAACAGCAGATTCGCCTGGATGACCAGAAAGAGCGAGGAAACCATCACCCCCTGGAACTTCGAGGAAGTGGTGGACAAGGGCGCTTCCGCCCAGAGCTTCATCGAGCGGATGACCAACTTCGATAAGAACCTGCCCAACGAGAAGGTGCTGCCCAAGCACAGCCTGCTGTACGAGTACTTCACCGTGTATAACGAGCTGACCAAAGTGAAATACGTGACCGAGGGAATGAGAAAGCCCGCCTTCCTGAGCGGCGAGCAGAAAAAGGCCATCGTGGACCTGCTGTTCAAGACCAACCGGAAAGTGACCGTGAAGCAGCTGAAAGAGGACTACTTCAAGAAAATCGAGTGCTTCGACTCCGTGGAAATCTCCGGCGTGGAAGATCGGTTCAACGCCTCCCTGGGCACATACCACGATCTGCTGAAAATTATCAAGGACAAGGACTTCCTGGACAATGAGGAAAACGAGGACATTCTGGAAGATATCGTGCTGACCCTGACACTGTTTGAGGACAGAGAGATGATCGAGGAACGGCTGAAAACCTATGCCCACCTGTTCGACGACAAAGTGATGAAGCAGCTGAAGCGGCGGAGATACACCGGCTGGGGCAGGCTGAGCCGGAAGCTGATCAACGGCATCCGGGACAAGCAGTCCGGCAAGACAATCCTGGATTTCCTGAAGTCCGACGGCTTCGCCAACAGAAACTTCATGCAGCTGATCCACGACGACAGCCTGACCTTTAAAGAGGACATCCAGAAAGCCCAGGTGTCCGGCCAGGGCGATAGCCTGCACGAGCACATTGCCAATCTGGCCGGCAGCCCCGCCATTAAGAAGGGCATCCTGCAGACAGTGAAGGTGGTGGACGAGCTCGTGAAAGTGATGGGCCGGCACAAGCCCGAGAACATCGTGATCGAAATGGCCAGAGAGAACCAGACCACCCAGAAGGGACAGAAGAACAGCCGCGAGAGAATGAAGCGGATCGAAGAGGGCATCAAAGAGCTGGGCAGCCAGATCCTGAAAGAACACCCCGTGGAAAACACCCAGCTGCAGAACGAGAAGCTGTACCTGTACTACCTGCAGAATGGGCGGGATATGTACGTGGACCAGGAACTGGACATCAACCGGCTGTCCGACTACGATGTGGACCATATCGTGCCTCAGAGCTTTCTGGCCGACGACTCCATCGACAACAAGGTGCTGACCAGAAGCGACAAGAACCGGGGCAAGAGCGACAACGTGCCCTCCGAAGAGGTCGTGAAGAAGATGAAGAACTACTGGCGGCAGCTGCTGAACGCCAAGCTGATTACCCAGAGAAAGTTCGACAATCTGACCAAGGCCGAGAGAGGCGGCCTGAGCGAACTGGATAAGGCCGGCTTCATCAAGAGACAGCTGGTGGAAACCCGGCAGATCACAAAGCACGTGGCACAGATCCTGGACTCCCGGATGAACACTAAGTACGACGAGAATGACAAGCTGATCCGGGAAGTGAAAGTGATCACCCTGAAGTCCAAGCTGGTGTCCGATTTCCGGAAGGATTTCCAGTTTTACAAAGTGCGCGAGATCAACAACTACCACCACGCCCACGACGCCTACCTGAACGCCGTCGTGGGAACCGCCCTGATCAAAAAGTACCCTGCGCTGGAAAGCGAGTTCGTGTACGGCGACTACAAGGTGTACGACGTGCGGAAGATGATCGCCAAGAGCGAGCAGGAAATCGGCAAGGCTACCGCCAAGTACTTCTTCTACAGCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGGCGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAGGAA TAG Xho1 CTCGAGGATGGGGAC TAGtgA GCCGGCC Not1 GCGGCCGC GCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTATTTTCATT AAGCTGCTTACATAGAACTCGCGGCGATTG Sph1 GCATGC CGCCTTAAAATTTTTATTTTATTTTTtCTTTTCTTTTCCGAATCGGATTTTGTTTTTAATATTTCAAAAAAAAAAAAAAAAAAAAAAAAACGCGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaatAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAttaattaa Xba1 tctaga acgcgTCGAGGGGAATTAATTCTTGAAGACGAAAGGGCCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATGCTTCAATAATATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTGTTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTCCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGAGCTC >210316-33_A11_1FC0DAA038.ab1 1225 3/17/21 2939 seq using VEE-seq-R NNNNNNNNNNNNNNGNNGCTTNTGNNTAAATGTTTTTTATTAGGCAGAAT CCAGATGCTCAAGGCCCTTCATAATATCCCCCAGTTTAGTAGTTGGACTT AGGGAACAAAGGAACCTTTAATAGAAATTGGACAGCAAGAAAGCGAGCGC GGCCGCGGCCGGCTCACTAGTCCCCATCCTCGAGCTATTCCTTTTTCTTT TTTGCCTGGCCGGCCTTTTTCGTGGCCGCCGGCCTTTTGTCGCCTCCCAG CTGAGACAGGTCGATCCGTGTCTCGTACAGGCCGGTGATGCTCTGGTGGA TCAGGGTGGCGTCCAGCACCTCTTTGGTGCTGGTGTACCTCTTCCGGTCG ATGGTGGTGTCAAAGTACTTGAAGGCGGCAGGGGCTCCCAGATTGGTCAG GGTAAACAGGTGGATGATATTCTCGGCCTGCTCTCTGATGGGCTTATCCC GGTGCTTGTTGTAGGCGGACAGCACTTTGTCCAGATTAGCGTCGGCCAGG ATCACTCTCTTGGAGAACTCGCTGATCTGCTCGATGATCTCGTCCAGGTA GTGCTTGTGCTGTTCCACAAACAGCTGTTTCTGCTCATTATCCTCGGGGG AGCCCTTCAGCTTCTCATAGTGGCTGGCCAGGTACAGGAAGTTCACATAT TTGGAGGGCAGGGCCAGTTCGTTTCCCTTCTGCAGTTCGCCGGCAGAGGC CAGCATTCTCTTCCGGCCGTTTTCCAGCTCGAACAGGGAGTACTTAGGCA GCTTGATGATCAGGTCCTTTTTCACTTCTTTGTAGCCCTTGGCTTCCAGA AAGTCGATGGGATTCTTCTCGAAGCTGCTTCTTTCCATGATGGTGATCCC CAGCAGCTCTTTCACACTCTTCAGTTTCTTGGACTTGCCCTTTTCCACTT TGGCCACCACCAGCACAGAATAGGCCACGGTGGGGCTGTCGAAGCCGCCG TACTTCTTAGGGTCCCAGTCCTTCTTTCTGGCGATCAGCTTATCGCTGTT CCTCTTGGGCAGGATAGACTCTTTGCTGAAGCCGCCTGTCTGCACCTCGG TCTTTTTCACGATATTCACTTGGGGCATGCTCAGCACTTTCCGCACGGTG GCAAAATCCCGGCCCTTATCCCACACGATCTCCCCGGTTTCGCCGTTTGT CTCGATCAGAGGCGCCTTCCGGATCTCGCCGTTGGCCAGGGTAATCTCGG TCTTGAAAAAGTTCATGAAGTTGCC rev comp GGCAACTTCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGGCGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATTTTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGGTGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGATCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGGCCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTGTGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCGACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTAAGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAACTGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCAGCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGGAACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAGTGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATAAGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAGCCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCAAAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGATCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGGCAAAAAAGAAAAAGGAATAGCTCGAGGATGGGGACTAGTGAGCCGGCCGCGGCCGCGCTCGCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAACTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTANNCANAAGCNNCNNNNNNNNNNNNNN Score Expect Identities Gaps Strand 2207 bits(1195) 0.0 1197/1198(99%) 0/1198(0%) Plus/Plus Query 2 GCAACTTCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGG 61 ||||| |||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3302 GCAACATCATGAACTTTTTCAAGACCGAGATTACCCTGGCCAACGGCGAGATCCGGAAGG 3361 Query 62 CGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATT 121 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3362 CGCCTCTGATCGAGACAAACGGCGAAACCGGGGAGATCGTGTGGGATAAGGGCCGGGATT 3421 Query 122 TTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGG 181 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3422 TTGCCACCGTGCGGAAAGTGCTGAGCATGCCCCAAGTGAATATCGTGAAAAAGACCGAGG 3481 Query 182 TGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGA 241 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3482 TGCAGACAGGCGGCTTCAGCAAAGAGTCTATCCTGCCCAAGAGGAACAGCGATAAGCTGA 3541 Query 242 TCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGG 301 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3542 TCGCCAGAAAGAAGGACTGGGACCCTAAGAAGTACGGCGGCTTCGACAGCCCCACCGTGG 3601 Query 302 CCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTG 361 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3602 CCTATTCTGTGCTGGTGGTGGCCAAAGTGGAAAAGGGCAAGTCCAAGAAACTGAAGAGTG 3661 Query 362 TGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCG 421 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3662 TGAAAGAGCTGCTGGGGATCACCATCATGGAAAGAAGCAGCTTCGAGAAGAATCCCATCG 3721 Query 422 ACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTA 481 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3722 ACTTTCTGGAAGCCAAGGGCTACAAAGAAGTGAAAAAGGACCTGATCATCAAGCTGCCTA 3781 Query 482 AGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAAC 541 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3782 AGTACTCCCTGTTCGAGCTGGAAAACGGCCGGAAGAGAATGCTGGCCTCTGCCGGCGAAC 3841 Query 542 TGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCA 601 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3842 TGCAGAAGGGAAACGAACTGGCCCTGCCCTCCAAATATGTGAACTTCCTGTACCTGGCCA 3901 Query 602 GCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGG 661 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3902 GCCACTATGAGAAGCTGAAGGGCTCCCCCGAGGATAATGAGCAGAAACAGCTGTTTGTGG 3961 Query 662 AACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAG 721 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 3962 AACAGCACAAGCACTACCTGGACGAGATCATCGAGCAGATCAGCGAGTTCTCCAAGAGAG 4021 Query 722 TGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATA 781 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4022 TGATCCTGGCCGACGCTAATCTGGACAAAGTGCTGTCCGCCTACAACAAGCACCGGGATA 4081 Query 782 AGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAG 841 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4082 AGCCCATCAGAGAGCAGGCCGAGAATATCATCCACCTGTTTACCCTGACCAATCTGGGAG 4141 Query 842 CCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCA 901 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4142 CCCCTGCCGCCTTCAAGTACTTTGACACCACCATCGACCGGAAGAGGTACACCAGCACCA 4201 Query 902 AAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGA 961 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4202 AAGAGGTGCTGGACGCCACCCTGATCCACCAGAGCATCACCGGCCTGTACGAGACACGGA 4261 Query 962 TCGACCTGTCTCAGCTGGGAGGCGACaaaaggccggcggccacgaaaaaggccggccagg 1021 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4262 TCGACCTGTCTCAGCTGGGAGGCGACAAAAGGCCGGCGGCCACGAAAAAGGCCGGCCAGG 4321 Query 1022 caaaaaagaaaaaGGAATAGCTCGAGGATGGGGACTAGTGAGCCGGCCGCGGCCGCGCTC 1081 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4322 CAAAAAAGAAAAAGGAATAGCTCGAGGATGGGGACTAGTGAGCCGGCCGCGGCCGCGCTC 4381 Query 1082 GCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAA 1141 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4382 GCTTTCTTGCTGTCCAATTTCTATTAAAGGTTCCTTTGTTCCCTAAGTCCAACTACTAAA 4441 Query 1142 CTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTA 1199 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 4442 CTGGGGGATATTATGAAGGGCCTTGAGCATCTGGATTCTGCCTAATAAAAAACATTTA 4499 >210316-33_G11_1FC0DAA039.ab1 1303 3/17/21 2939 seq using EFC-F1 CatatgaagctTAATACGACTCACTATAagGagaataaac good NNNNTNNNNNNNNNNNACTAGTATTCTTCTGGTCCCCACAGACTCAGAGA GAACCGCCACCATGGACTATAaGGACCACGACGGAGACTACAAGGATCAT GATATTGATTACAAAGACGATGACGATAAGATGGCCCCAAAGAAGAAGCG GAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGCATCG GCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAG TACAAGGTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCA CAGCATCAAGAAGAACCTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAA CAGCCGAGGCCACCCGGCTGAAGAGAACCGCCAGAAGAAGATACACCAGA CGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTCAGCAACGAGATGGC CAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTGGTGG AAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGAC GAGGTGGCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAA ACTGGTGGACAGCACCGACAAGGCCGACCTGCGGCTGATCTATCTGGCCC TGGCCCACATGATCAAGTTCCGGGGCCACTTCCTGATCGAGGGCGACCTG AACCCCGACAACAGCGACGTGGACAAGCTGTTCATCCAGCTGGTGCAGAC CTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTGGACG CCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAAT CTGATCGCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCT GATTGCCCTGAGCCTGGGCCTGACCCCCAACTTCAAGAGCAACTTCGACC TGGCCGAGGATGCCAAACTGCAGCTGAGCAAGGACACCTACGACGACGAC CTGGACAACCTGCTGGCCCAGATCGGCGACCAGTACGCCGACCTGTTTCT GGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTGAAAG TGAACACCGAGATCACCAGGGCCCCCCTGAACGCCTCTATGATCAGNNAT ACCAACAAGCACCACCAGGACCTGACCCTGCTGAAAGNNCTCGNGCGGAA ACNNNTGCCTGAGAANNNCAANNGATTTTCTTNCANNNAANNAGAAACGG CNACCCCGGTTNCTTNNNNNGNGGNCCNNCCNGAAAAATTCTCNNTNNNN TGC Score Expect Identities Gaps Strand 2132 bits(1154) 0.0 1182/1200(99%) 2/1200(0%) Plus/Plus Query 17 ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCGCCACCATGGACTATAaGGAC 76 ||||||||||||||||||||||||||||||||||||||||||||||||||||||| |||| Sbjct 22 ACTAGTATTCTTCTGGTCCCCACAGACTCAGAGAGAACCGCCACCATGGACTATAAGGAC 81 Query 77 CACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCC 136 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 82 CACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGATGGCC 141 Query 137 CCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGC 196 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 142 CCAAAGAAGAAGCGGAAGGTCGGTATCCACGGAGTCCCAGCAGCCGACAAGAAGTACAGC 201 Query 197 ATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAG 256 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 202 ATCGGCCTGGACATCGGCACCAACTCTGTGGGCTGGGCCGTGATCACCGACGAGTACAAG 261 Query 257 GTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAAC 316 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 262 GTGCCCAGCAAGAAATTCAAGGTGCTGGGCAACACCGACCGGCACAGCATCAAGAAGAAC 321 Query 317 CTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGA 376 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 322 CTGATCGGAGCCCTGCTGTTCGACAGCGGCGAAACAGCCGAGGCCACCCGGCTGAAGAGA 381 Query 377 ACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTC 436 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 382 ACCGCCAGAAGAAGATACACCAGACGGAAGAACCGGATCTGCTATCTGCAAGAGATCTTC 441 Query 437 AGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTG 496 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 442 AGCAACGAGATGGCCAAGGTGGACGACAGCTTCTTCCACAGACTGGAAGAGTCCTTCCTG 501 Query 497 GTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTG 556 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 502 GTGGAAGAGGATAAGAAGCACGAGCGGCACCCCATCTTCGGCAACATCGTGGACGAGGTG 561 Query 557 GCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACC 616 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 562 GCCTACCACGAGAAGTACCCCACCATCTACCACCTGAGAAAGAAACTGGTGGACAGCACC 621 Query 617 GACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGC 676 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 622 GACAAGGCCGACCTGCGGCTGATCTATCTGGCCCTGGCCCACATGATCAAGTTCCGGGGC 681 Query 677 CACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATC 736 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 682 CACTTCCTGATCGAGGGCGACCTGAACCCCGACAACAGCGACGTGGACAAGCTGTTCATC 741 Query 737 CAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTG 796 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 742 CAGCTGGTGCAGACCTACAACCAGCTGTTCGAGGAAAACCCCATCAACGCCAGCGGCGTG 801 Query 797 GACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATC 856 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 802 GACGCCAAGGCCATCCTGTCTGCCAGACTGAGCAAGAGCAGACGGCTGGAAAATCTGATC 861 Query 857 GCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTG 916 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 862 GCCCAGCTGCCCGGCGAGAAGAAGAATGGCCTGTTCGGAAACCTGATTGCCCTGAGCCTG 921 Query 917 GGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTG 976 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 922 GGCCTGACCCCCAACTTCAAGAGCAACTTCGACCTGGCCGAGGATGCCAAACTGCAGCTG 981 Query 977 AGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTAC 1036 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 982 AGCAAGGACACCTACGACGACGACCTGGACAACCTGCTGGCCCAGATCGGCGACCAGTAC 1041 Query 1037 GCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTG 1096 |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| Sbjct 1042 GCCGACCTGTTTCTGGCCGCCAAGAACCTGTCCGACGCCATCCTGCTGAGCGACATCCTG 1101 Query 1097 AAAGTGAACACCGAGATCACCAGGGCCCCCCTGAACGCCTCTATGATCA-GNNATACCAA 1155 | |||||||||||||||||||| ||||||||||| |||||||||||||| | |||| | Sbjct 1102 AGAGTGAACACCGAGATCACCAAGGCCCCCCTGAGCGCCTCTATGATCAAGAGATACG-A 1160 Query 1156 CAAGCACCACCAGGACCTGACCCTGCTGAAAGNNCTCGNGCGGAAACNNNTGCCTGAGAA 1215 | |||||||||||||||||||||||||||||| |||| |||| | | |||||||||| Sbjct 1161 CGAGCACCACCAGGACCTGACCCTGCTGAAAGCTCTCGTGCGGCAGCAGCTGCCTGAGAA 1220 </pre> [[Category:Cloning]]
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