Editing 3010 pCMV5-Flag-kappa

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http://www.excellgen.net/eln/images/2/2f/3001_3010_pMiniT2_digestion.JPG



2/4/2018

3010 pCMV5-Flag-kappa, Flag light<br/>need to fix from 3135-4-pMiniT2 >as-F_E12.ab1 template<br/>it is actually 3010: missing&nbsp;<br/>5' end ATCATCGGATCCGCC<br/>3' end: GGAGGAACAAAGCTCGAGATCAAGCGGGCTAGCGGTACCATCATC<br/>using these oligos<br/>1 ul of 10 uM<br/>3010-Flag-VL-1C4ATCATCGGATCCGCCGCCACCATG<br/>1 ul of 1 uM<br/>3010-Flag-VL-2C5AGAGCAGCAGGAGCCCCAGGAGCTGAGCGGGGACCCTCATGTCCATGGTGGCGGCGGA<br/>1 ul of 1 uM<br/>3010-Flag-VL-11D2CGCTCCTTACACCTTTGGCGGAGGAACAAAGCTCGAGATCAAGCGGGCTAGCGGTACC<br/>1 ul of 10 uM<br/>3010-Flag-VL-12D3GATGATGGTACCGCTAGCCCGC<br/>3135-4-pMiniT2 as template

<br/>Vector Name: 2493 pCMV5-mIgG-kLC<br/>Restriction Enzymes: BamH1-Kpn1

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'''3010 miniprep:'''

Restriction enzymes: BamHI+KpnI

Time:15min

Buffer:Fastdigest

'''Result:good, sent to do sequencing.#4'''

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