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359-pET30-pMBP
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Cloning of 359-pET30-pMBP<br> Purpose: to clone pET30-pMBP by PCR Date: 2011-10-4---- Notebook:Lu yingying Page: Clone ID:359 Experimenters: lu yingying<br>Results<br> [[Image:359-pMBP-PCR-product10-4-2011.JPG]]<br> PCR product of pMBP<br> template: 424-pDEST-periHisMBP<br> primer:periMBP-F + 10% periMBP-R1 + periMBP-R2<br> 25 cycles [[Image:359pMBP+PET30b digest 10-5-2011.JPG]]<br> digest result:NdeI + NcoI<br> band 1 shows pMBP digest result, band 2 shows PET30b digest result<br> [[Image:344,359,391+393digest detect10-7-2011.JPG|519x241px|344,359,391+393digest detect10-7-2011.JPG]] 359,1-6 bands show digest result: no insert <br> Cloning of 359-pET30-pMBP Purpose: to clone pET30-pMBP by PCR Date: 2011-10-17---- Notebook:Lu yingying Page: Clone ID:359 Experimenters: lu yingying<br>Results<br> [[Image:359-PMBP10-17-2011.JPG]]<br> PCR product of pMBP template: 424-pDEST-periHisMBP primer:periMBP-F + 10% periMBP-R1 + periMBP-R2 25 cycles <br> [[Image:359-PMBP digest10-17-2011.JPG]]<br> digest result:NdeI + NcoI<br> [[Image:359-PET30b digest-10-17-2011.JPG]]<br> digest result:NdeI + NcoI<br> ligation RT overnight <br> Cloning of 359-pET30-pMBP Purpose: to clone pET30-pMBP by PCR Date: 2011-10-19---- Notebook:Lu yingying Page: Clone ID:359 Experimenters: lu yingying<br>Results<br> [[Image:359-PMBP PCR product10-19-2011.JPG]]<br> <br> <br> <br> [[Image:359-vector+pMBP digest10-20-2011.JPG]]<br> digest result:NdeI + NcoI NEB enzyme for 2 hours<br> <br> Date: 2011-10-25---- Notebook:Lu yingying Page: Clone ID:359 Experimenters: lu yingying<br>Results<br> [[Image:359-PMBP PCR product10-19-2011.JPG]]<br> PCR product of pMBP template: 424-pDEST-periHisMBP primer:periMBP-F + 10% periMBP-R1 + periMBP-R2 25 cycles <br> 10-19 PCR product 10ul left<br> [[Image:359 subclone 10-26-2011.JPG]]<br> digest result:NdeI + NcoI <br> [[Image:414+470+375+359 digest detect10-27-2011.JPG]]<br> 359 digest detect: NdeI + NcoI<br> <br> <br> Date: 2011-11-4----2011-11-7 Notebook:Lu yingying Page:003966----003971 Clone ID:359 Experimenters: lu yingying<br>Results<br> [[Image:359-PMBP PCR product10-19-2011.JPG]]<br> PCR product of pMBP template: 424-pDEST-periHisMBP primer:periMBP-F + 10% periMBP-R1 + periMBP-R2 25 cycles 10-19 PCR product 10ul left<br> [[Image:375+359_clone_11-4-2011.JPG]]<br> digest result:NdeI + NcoI <br> [[Image:359+375_digest_detect11-7-2011.JPG]]<br> 5 clonies digest detect:NdeI + NcoI ,need to sequence to detect <br> <br> <br> <br> <br> <br> <br> [[Category:Cloning]]
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