Editing 414-pAd5-CMV-Cre

Cloning of pAd5-CMV-Cre<br> 

Purpose: to clone pAd5-CMV-Cre by subclone from241 pLentiGP-Cre to 280 pAd5-CMV-MCS<br> 

Date: 2011-9-29 

Notebook:Lu yingying 

Page: 

Clone ID:414 

Experimenters: lu yingying<br>Results<br> 

[[Image:256,414 subclone9-29-2011.JPG]] 

bands 3 show cre insert ,bands 4 show pAd5 vector 

Ligation at RT for 2h 

[[Image:256,414,417digest detect10-3-2011.JPG|457x225px|256,414,417digest detect10-3-2011.JPG]]<br> 

digest detect:EcoR1+Xho1<br> 

<br> 

Date: 2011-10-25 

Notebook:Lu yingying 

Page: 

Clone ID:414 

Experimenters: lu yingying<br>Results<br> 

[[Image:414+375 subclone digest10-25-2011.JPG]]<br> 

band 1,2 show subclone digest result:EcoR1+Xho1<br> 

241 pLentiGP-Cre to 280 pAd5-CMV-MCS, band 1 show 241 digest result,band 2 show 280 digest result<br> 

[[Image:414+470+375+359 digest detect10-27-2011.JPG|494x270px|414+470+375+359 digest detect10-27-2011.JPG]]<br> 

band 414,1-6 show digest detect result:EcoR1+Xho1<br> 

<br> 

<br> 

== '''Cloning of pAd5-CMV-Cre'''  ==

'''Purpose:''' Cloning of pAd5-CMV-Cre&nbsp; from241 pLentiGP-Cre to 280 pAd5-CMV-MCS 

'''Date: '''2011-12-14--- 

'''Notebook:''' 

'''Page:''' 

'''Clone ID: 414''' 

'''Experimenters:''' yingying 

== '''Results'''  ==

=== Subcloning  ===

Date: 2011-12-14--- 

[[Image:414,454 Clone 12-14-2011.JPG]]<br> 

{| cellspacing="1" cellpadding="1" border="1" style="width: 301px; height: 80px;"
|-
| 414<br> 
| band1<br> 
| band2<br>
|-
| <br> 
| 241 pLentiGP-Cre<br> 
| 280 pAd5-CMV-MCS<br>
|-
| digest<br> 
| EcoR1+Xho1<br> 
| EcoR1+Xho1<br>
|}

<br> 

<br> 

=== Colony Screening  ===

Date: 2011-12-16<br> 

Make miniprep and digest with EcoR1+Xho1<br> 

&nbsp;[[Image:414+454 digest detect 12-16-2011.JPG]]<br> 

lanes 1,2,3,5,6,7 show right, need to sequence<br> 

== '''Result intepretation/Summary'''  ==

*Sequencing Result:414-6 right<br>

== '''Conclusion''' ==

414-6 right
<br> <br>  ==

===  ===

<br> --[[User:Yingying.lu|Yingying.lu]] 08:47, 19 December 2011 (EST) 

[[Category:Cloning]]