Editing Baculovirus Cre FLP vlpQC cre QC 20141103

VLP from sf9 cells. 

Original FLP: VLP in sf9 cell culture medium<br> 

Final Purified FLP: Concentrated from 1L to to 200ml (FLP) by ultrafiltration, purified by gel filtration, then ultrafiltrated again, filtered on 10/10/2014<br> 

<br> 

Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration , filtered on 10/08/2014<br> 

Final Purified Cre: Concentrated from 1L to to 200ml (FLP) by ultrafiltration , dialyzed , purified by gel filtration ,then ultrafiltrated again, filtered on 10/10/2014<br> 

<br> 

Reporter: 2013 for Flp, 2181 for Cre<br> 

<br> 

Oct. 30 2014 Morning&nbsp;: split 293 cells<br> 

Oct. 30 2014 afternoon Transfect 293 cell (70--80%) using lipofectamin 2000<br> 

Oct.31 2014 10:00 am: 70%-80% cells&nbsp; turns red , cells are in good condition, add Flp and&nbsp; Cre to reporter cells<br> 

{| width="1216" cellspacing="1" cellpadding="1" border="1"
|-
| 
A1: <br> 

75ul original FLP 

| 
A2:<br> 

50ul Final Purified FLP

(filtered on 10/10/2014)

| <br>
| 
A3:<br> 

75ul Purified cre 

(filtered on 10/8/2014)<br>

| 
A4:<br> 

50 ul Final Purified FLP

(filtered on 10/10/2014)

| <br>
| 
A5:<br> 

1uM cre<br>

lot#: 20130826<br>

| 
A6:<br> 

2.5uM cre<br>

lot#: 20130826<br>

|-
| B1: 
0ul Final Purified FLP<br> 

(filtered on 10/24/2014)<br> 

| B2: 
75ul Final Purified FLP<br> 

(filtered on 10/242014)<br> 

| <br>
| B3: 
0ul Final Purified FLP<br> 

(filtered on 10/29/2014)<br> 

| B4: 
50ul Final Purified FLP <br> 

(filtered on 10/10/2014)<br> 

| <br>
| 
B5:

1uM cre

lot#: 20131115<br>

| 
B6:

2.5uM cre

lot#: 20131115<br>

|-
| 
C1: 

100ul Final Purified FLP 

(filtered on 10/24/2014)<br> 

| C2: 
150ul Final Purified FLP<br> 

(filtered on 10/24/2014)<br> 

| <br>
| C3: 
75ul Final Purified FLP<br> 

(filtered on 10/29/2014)<br><br> 

| C4: 
100ul Final Purified Cre<br> 

(filtered on 10/29/2014)<br> 

| <br>
| C5:
1uM cre

lot#: 20131219<br> 

| C6:
2.5uM cre

lot#: 20131219<br> 

|-
| D1:
200ul Final Purified FLP

(filtered on 10/24/2014)<br>

| D2:
250ul Final Purified FLP

(filtered on 10/24/2014)<br>

| <br>
| D3:
150ul Final Purified FLP

(filtered on 10/29/2014)<br><br>

| D4:
150ul Final Purified FLP

(filtered on 10/29/2014)<br><br>

| <br>
| 
D5:

1uM cre

lot#: 56375<br>

| 
D6:

2.5uM cre

lot#: 56375<br>

|}

Nov.3 

[[Image:20141103cre flp vlp creqc.jpg]]<br>

<br> 

[[Category:QC]]