Editing Humanization of the antibodies by germline-humanization

Humanization of the macaque antibodies by germline-humanization<br/>The sequence of each antibody was compared with the human germline genes using the<br/>IMGT/V-QUEST online tool from the International ImmunoGeneTics information system1<br/>(IMGT) (http://www.imgt.org). This tool allows identification of the human germline genes<br/>most similar to any given variable region and calculates the Germinality Index (GI), defined as<br/>the percentage identity between a given framework region (FR) and the most similar human<br/>germline sequence. Based on the physiochemical classes of the amino acids (AA), differences<br/>in the FRs were classified as very similar, similar, dissimilar and very dissimilar AA. Firstly,<br/>humanized variants of VH and VL were designed by exchanging the very similar AA and similar<br/>AA followed by dissimilar AA and very dissimilar AA. The structure of each domain was<br/>modeled using WAM antibody modeling [33] and visualized using UCSF Chimera software<br/>[45]. The humanized VH and VL genes were synthesized by GeneArt1 Gene Synthesis<br/>(Regensburg, Germany) and were used for expression of scFv-Fc and IgG.
[[Category:Antibodies]]