Editing NEB Competent Cells

NEB Competent Cells 

{| cellspacing="0" cellpadding="1" border="0" class="tableborder"
|-
| nowrap="nowrap" colspan="17" | Cloning Strain Properties
|-
| nowrap="nowrap" bgcolor="#c8d3a0" align="center" style="font-weight: bold;" | Strain Properties 
| bgcolor="#c8d3a0" style="font-weight: bold;" | NEB# 
| bgcolor="#c8d3a0" style="font-weight: bold;" | Transform<br> 
Efficiency<br> (cfu/µg)(1) 

| bgcolor="#c8d3a0" style="font-weight: bold;" | Strain 
| bgcolor="#c8d3a0" style="font-weight: bold;" | T1<br> 
Phage<br> Resistant 

| bgcolor="#c8d3a0" style="font-weight: bold;" | Library<br> 
Construc<br> -tion(2) 

| bgcolor="#c8d3a0" style="font-weight: bold;" | Cloning/<br> 
Sub<br> cloning 

| bgcolor="#c8d3a0" style="font-weight: bold;" | Blue/<br> 
White<br> Screening 

| bgcolor="#c8d3a0" | laclq 
| bgcolor="#c8d3a0" | lysY 
| bgcolor="#c8d3a0" style="font-weight: bold;" | Endo-<br> 
nucleaseI<br> Deficient(3) 

| bgcolor="#c8d3a0" style="font-weight: bold;" | Protease(4)<br> 
Deficient 

| bgcolor="#c8d3a0" style="font-weight: bold;" | F´ 
| bgcolor="#c8d3a0" style="font-weight: bold;" | RecA¯ 
| bgcolor="#c8d3a0" style="font-weight: bold;" | T7 RNA<br> 
Polyme<br> -rase 

| bgcolor="#c8d3a0" style="font-weight: bold;" | Cytoplasmic<br> 
disulfide bond<br> formation(5) 

| bgcolor="#c8d3a0" style="font-weight: bold;" | Drug<br> 
Resistance(6) 

|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2984.asp NEB Turbo] 
| C2984 
| 1-3x109 
| K12 
| √ 
| √ 
| √ 
| √ 
| √ 
| – 
| √ 
| – 
| √ 
| – 
| – 
| – 
| nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2987.asp NEB 5-alpha] 
| bgcolor="#d4d4d4" | C2987 
| bgcolor="#d4d4d4" | 1-3x109 
| bgcolor="#d4d4d4" | K12 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | none
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2992.asp NEB 5-alpha F'Iq] 
| C2992 
| 1-3x109 
| K12 
| √ 
| √ 
| √ 
| √ 
| √ 
| – 
| √ 
| – 
| √ 
| √ 
| – 
| – 
| tet
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3019.asp NEB 10-beta] 
| bgcolor="#d4d4d4" | C3019 
| bgcolor="#d4d4d4" | 1-3x109 
| bgcolor="#d4d4d4" | K12 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | str
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2925.asp dam-/dcm-] 
| C2925 
| 1-3x106 
| K12 
| √ 
| √ 
| √ 
| – 
| – 
| – 
| √ 
| – 
| – 
| – 
| – 
| – 
| cam, str, nit
|-
| nowrap="nowrap" | &nbsp; 
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| &nbsp; 
| &nbsp; 
| &nbsp; 
| &nbsp; 
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| &nbsp; 
| &nbsp; 
| &nbsp; 
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| nowrap="nowrap" colspan="17" | Protein Expression Strain Properties
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2523.asp NEB Express] 
| bgcolor="#d4d4d4" | C2523 
| bgcolor="#d4d4d4" | 0.6-1x109 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3037.asp NEB Express Iq] 
| C3037 
| 0.6-1x109 
| B 
| √ 
| √ 
| – 
| – 
| √ 
| – 
| √ 
| √ 
| – 
| – 
| – 
| – 
| cam, nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2566.asp T7 Express] 
| bgcolor="#d4d4d4" | C2566 
| bgcolor="#d4d4d4" | 0.6-1x109 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3016.asp T7 Express Iq] 
| C3016 
| 0.6-1x109 
| B 
| √ 
| √ 
| – 
| – 
| √ 
| – 
| √ 
| √ 
| – 
| – 
| √ 
| – 
| cam, nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3010.asp T7 Express lysY] 
| bgcolor="#d4d4d4" | C3010 
| bgcolor="#d4d4d4" | 0.6-1x109 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | cam, nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3013.asp T7 Express lysY/Iq] 
| C3013 
| 0.6-1x109 
| B 
| √ 
| √ 
| – 
| – 
| √ 
| √ 
| √ 
| √ 
| – 
| – 
| √ 
| – 
| cam, nit
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3022.asp T7 Express Crystal] 
| bgcolor="#d4d4d4" | C3022 
| bgcolor="#d4d4d4" | 0.6-1x109 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | nit
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3025.asp SHuffle] 
| C3025 
| 1 x 106 
| K12 
| √ 
| – 
| – 
| – 
| √ 
| – 
| – 
| – 
| √ 
| – 
| – 
| √ 
| str, spec
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3026.asp SHuffle T7] 
| bgcolor="#d4d4d4" | C3026 
| bgcolor="#d4d4d4" | 1 x 106 
| bgcolor="#d4d4d4" | K12 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | str, spec
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3027.asp SHuffle T7 lysY] 
| C3027 
| 1 x 106 
| K12 
| √ 
| – 
| – 
| – 
| √ 
| √ 
| – 
| – 
| – 
| – 
| √ 
| √ 
| cam, str, spec
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3028.asp SHuffle Express] 
| bgcolor="#d4d4d4" | C3028 
| bgcolor="#d4d4d4" | 1 x 107 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | spec
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC3029.asp SHuffle T7 Express] 
| C3029 
| 1 x 107 
| B 
| √ 
| – 
| – 
| – 
| √ 
| – 
| √ 
| √ 
| – 
| – 
| √ 
| √ 
| spec(7)
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC3030.asp SHuffle T7 Express lysY] 
| bgcolor="#d4d4d4" | C3030 
| bgcolor="#d4d4d4" | 1 x 107 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | cam,<br> 
spec(7) 

|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2530.asp BL21] 
| C2530 
| 1-5x107 
| B 
| √ 
| – 
| – 
| – 
| – 
| – 
| – 
| √ 
| – 
| – 
| – 
| – 
| –
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2527.asp BL21(DE3)] 
| bgcolor="#d4d4d4" | C2527 
| bgcolor="#d4d4d4" | 1-5x107 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | –
|-
| nowrap="nowrap" | [http://www.neb.com/nebecomm/products/productC2529.asp NiCo21(DE3)] 
| C2529 
| 1-5x107 
| B 
| √ 
| – 
| – 
| – 
| – 
| – 
| – 
| √ 
| – 
| – 
| √ 
| – 
| –
|-
| nowrap="nowrap" bgcolor="#d4d4d4" | [http://www.neb.com/nebecomm/products/productC2528.asp Lemo21(DE3)] 
| bgcolor="#d4d4d4" | C2528 
| bgcolor="#d4d4d4" | 1-3x107 
| bgcolor="#d4d4d4" | B 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | √ 
| bgcolor="#d4d4d4" | – 
| bgcolor="#d4d4d4" | cam
|}

(1) TE given are for high efficiency chemically competent strains. TE for electrocompetent strains are 1-4 x 1010cfu/mg. TE for subcloning strains are &gt;1 x 106.<br> 

(2) Restriction-deficient strain.<br> (3) Important for plasmid preparation.<br> (4) Lacks Lon and OmpT protease activity.<br> (5) Constitutively expresses a chromosomal copy of the disulfide bond isomerase DsbC.<br> (6) nit = nitrofurantoin, tet = tetracycline, cam = chloramphenicol, str = streptomycin, spec = spectinomycin<br> 

(7) Resistance to low levels of streptomycin may be observed. 

[[Category:Protein_Expression]] [[Category:Competent_Cells]]