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Oligos for cloning antibody
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== Mouse B Cells or Hybridoma == <pre>Primers VL-for d V(μl) original outer-for: ctacagcaggcccagccggccatggcggactacaaaG L Q Q A Q P A M A D Y K ctacagcaggcccagccggccatggcggacgcgcgcGa L Q Q A Q P A M A D A R MKYLLPTAAAGLLLLAAQPA ATGAAATACCTATTGCCTACGGCGGCCGCTGGATTGTTATTACTCGCGGCCCAGCCGGCCATGGCCgcgcgcGA M K Y L L P T A A A G L L L L A A Q P A M A A R original 19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC VL-forκ1 catggcggactacaaaGACAWTGTTCTCACCCAGTC 2 6 -- 3 TCAATAGTTGAACATAGCGGCCGCA SAAAWTGTKCTCACCCAGTC κ light chains F3 VL-forκ2 catggcggactacaaaGACATCCAGATGACACAGWC 2 6 VL-forκ3 catggcggactacaaaGATRTTGTGATGACCCAGWC 4 6 --9 TCAATAGTTGAACATAGCGGCCGCA GATATCCAGATGACACAGAC κ light chains F9 --13 TCAATAGTTGAACATAGCGGCCGCA GAYATTGTGATGACBCAGKC κ light chains F13 VL-forκ4 catggcggactacaaaGACATTSTGMTGACCCAGTC 4 6 --18 TCAATAGTTGAACATAGCGGCCGCA GACATTRTGATGWCACAGTC κ light chains F18 --16 TCAATAGTTGAACATAGCGGCCGCA GAAAWTGTGYTGACCCAGTC κ light chains F16 --15 TCAATAGTTGAACATAGCGGCCGCA GACATCYTGCTGACYCAGTC κ light chains F15 --17 TCAATAGTTGAACATAGCGGCCGCA GAAACAACTGTGACCCAGTC κ light chains F17 VL-forκ5 catggcggactacaaaGATGTTGTGVTGACCCAAAC 3 6 --12 TCAATAGTTGAACATAGCGGCCGCA GATRTTKTGATGACYCARAC κ light chains F12 GATGTTTTGATGACCCAAACTCCA 2002 Fab VκA VL-forκ6 catggcggactacaaaGACACAACTGTGACCCAGTC 1 3 --19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC κ light chains F19 VL-forκ7 catggcggactacaaaGAYATTKTGCTCACTCAGTC 4 6 -- 4 TCAATAGTTGAACATAGCGGCCGCA GAWATTGTGCTMACTCAGTC κ light chains F4 VL-forκ8 catggcggactacaaaGATATTGTGATRACCCAGGM 4 6 note: VL-forκ8 was ordered from IDT as catggcggactacaaaGATATTGTGATRACCCAGGC maybe should be catggcggactacaaaGATATTGTGATRACCCAGGA -- 14 TCAATAGTTGAACATAGCGGCCGCA GATATTGTGATAACCCAGGA κ light chains F14 VL-forκ8 catggcggactacaaaGATATTGTGATRACCCAGGC 4 6 modified VL-forκ9 catggcggactacaaaGACATTGTAATGACCCAATC 1 3 -- 10 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTGACCCAATC κ light chains F10 -- 11 TCAATAGTTGAACATAGCGGCCGCA GACATYSTRATGACCCARTC κ light chains F11 GACATTGTGCTCACCCAATCTCC 2002 Fab VκB VL-forκ10 catggcggactacaaaGACATTGTGATGWCACAGTC 2 6 --6 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGATGACMCAGTC κ light chains F6 GACATTGTGCTRACCCAGTCTTCCA VκC Fab VκB -- 5 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTRACACAGTC κ light chains F5 VL-forκ11 catggcggactacaaaGATRTCCAGATGAMCCAGTC 4 6 -- 7 TCAATAGTTGAACATAGCGGCCGCA GAYATCMAGATRAMCCAGTC κ light chains F7 --8 TCAATAGTTGAACATAGCGGCCGCA GAYATCCAGATGAYTCAGTC κ light chains F8 VL-forκ12 catggcggactacaaaGATGGAGAAACAACACAGGC 1 3 optional VL-forλ1 catggcggactacaaaGACGCTGTTGTGACTCAGGA 1 1 VL-forλ2 catggcggactacaaaGACCYTGTGCTCACTCAGTC 2 2 my design outer-for: TTATTACTCGCGGCCCAGCCGGCCATGGCCgcgcgcGA VL-forκ1 GGCCATGGCCgcgcgcGACAWTGTTCTCACCCAGTC 2 6 VL-forκ2 GGCCATGGCCgcgcgcGACATCCAGATGACACAGWC 2 6 VL-forκ3 GGCCATGGCCgcgcgcGATRTTGTGATGACCCAGWC 4 6 VL-forκ4 GGCCATGGCCgcgcgcGACATTSTGMTGACCCAGTC 4 6 VL-forκ5 GGCCATGGCCgcgcgcGATGTTGTGVTGACCCAAAC 3 6 VL-forκ6 GGCCATGGCCgcgcgcGACACAACTGTGACCCAGTC 1 3 VL-forκ7 GGCCATGGCCgcgcgcGAYATTKTGCTCACTCAGTC 4 6 VL-forκ8 GGCCATGGCCgcgcgcGATATTGTGATRACCCAGGM 4 6 VL-forκ9 GGCCATGGCCgcgcgcGACATTGTAATGACCCAATC 1 3 VL-forκ10 GGCCATGGCCgcgcgcGACATTGTGATGWCACAGTC 2 6 VL-forκ11 GGCCATGGCCgcgcgcGATRTCCAGATGAMCCAGTC 4 6 VL-forκ12 GGCCATGGCCgcgcgcGATGGAGAAACAACACAGGC 1 3 optional VL-forλ1 GGCCATGGCCgcgcgcGACGCTGTTGTGACTCAGGA 1 1 VL-forλ2 catggcggacgcgcgcGACCYTGTGCTCACTCAGTC 2 2 Primers VL-rev 5' (Gly4Ser)3-linker | VL 3' VL-revκ1 ggagccgccgccgcc (agaaccaccaccacc)2 GCGTTTBATTTCCAGCTTGG 3 25.3 ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCGTTTBATTTCCAGCTTGG (CCAAGCTGGAAATVAAACGC ggtggtggtggttct ggtggtggtggttct ggcggcggcggctcc) K L E I/L K R G G G G S G G G G S G G G G S VL-revκ2 ggagccgccgccgcc (agaaccaccaccacc)2 GCGTTTTATTTCCAATTTTG 1 12.7 ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCGTTTTATTTCCAATTTTG optional VL-revλ ggagccgccgccgcc (agaaccaccaccacc)2 GCCTAGGACAGTCAMCYTGG 4 2 ggagccgccgccgccagaaccaccaccaccagaaccaccaccaccGCCTAGGACAGTCAMCYTGG Primers VH-for 5' (Gly4Ser)2-linker BamHI | VH 3' VH-for1 ggcggcggcggctccggtggtggt ggatcc GAGGTTCDSCTGCAACAGTY 12 4 G G G G S G G G G S ggcggcggcggctccggtggtggt ggatcc GAGGTTCDSCTGCAACAGTC modified -- 30 TTCACGAGTCCAGCCTCAAGCAGT GAGRTCCAGCTGCAACARTC ---VH primer F30 -- 33 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGMAGCTGGTGGAATC ---VH primer F33 VH-for2 ggcggcggcggctccggtggtggt ggatcc CAGGTGCAAMTGMAGSAGTC 8 3 VH-for3 ggcggcggcggctccggtggtggt ggatcc GAVGTGMWGCTGGTGGAGTC 12 4 -- 35 TTCACGAGTCCAGCCTCAAGCAGT GARGTGCAGCTGKTGGAGWC ---VH primer F35 VH-for4 ggcggcggcggctccggtggtggt ggatcc CAGGTTAYTCTGAAAGAGTC 2 2 VH-for5 ggcggcggcggctccggtggtggt ggatcc GAKGTGCAGCTTCAGSAGTC 2 2 --39 TTCACGAGTCCAGCCTCAAGCAGT GAAGTGAARMTTGAGGAGTC -----VH primer F39 --34 TTCACGAGTCCAGCCTCAAGCAGT GAVGTGMWGCTSGTGGAGTC -----VH primer F34 VH-for6 ggcggcggcggctccggtggtggt ggatcc CAGATCCAGTTSGYGCAGTC 4 2 -- 43 TTCACGAGTCCAGCCTCAAGCAGT CAGATCCAGTTSGTRCAGTC -----VH primer F43 ---31 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYVAGCTGCAGCAGTC -----VH primer F31 --Q_mVH_F6 CGTGACCACAGGCGTCCACAGC GACACAACTGTGACCCAGTC 2---- VH-for7 ggcggcggcggctccggtggtggt ggatcc CAGRTCCAACTGCAGCAGYC 4 2 VH-for8 ggcggcggcggctccggtggtggt ggatcc GAGGTGMAGCTASTTGAGWC 8 3 --38 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTTCTCRAGTC ---VH primer F38 --37 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGCAGCTTGTTGAGTC ---VH primer F37 --36 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTGATGGAATC ---VH primer F36 VH-for9 ggcggcggcggctccggtggtggt ggatcc GAAGTGAAGMTTGAGGAGTC 2 2 --27 TTCACGAGTCCAGCCTCAAGCAGT GAKRTRCAGCTTMAGGAGTC ---VH primer F27 VH-for10 ggcggcggcggctccggtggtggt ggatcc GATGTGAACCTGGAAGTGTC 1 1 -- 42 TTCACGAGTCCAGCCTCAAGCAGT GATGTGAACCTGGAAGTGTC ---VH primer F42 VH-for11 ggcggcggcggctccggtggtggt ggatcc CAGATKCAGCTTMAGGAGTC 4 2 VH-for12 ggcggcggcggctccggtggtggt ggatcc CAGGCTTATCTGCAGCAGTC 1 1 -- 40 TTCACGAGTCCAGCCTCAAGCAGT CAGGTTACTCWGAAAGWGTCTG ---VH primer F40 --29 TTCACGAGTCCAGCCTCAAGCAGT CAGGTGCAGMTGAAGSAGTC ---VH primer F29 --28 TTCACGAGTCCAGCCTCAAGCAGT GAGKTYCAGCTBCAGCAGTC ---VH primer F28 VH-for13 ggcggcggcggctccggtggtggt ggatcc CAGGTTCACCTACAACAGTC 1 1 --41 TTCACGAGTCCAGCCTCAAGCAGT CAGGTCCAACTGCAGCAGCC ---VH primer F41 --32 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYCARCTGCAGCAGTC ---VH primer F32 VH-for14 ggcggcggcggctccggtggtggt ggatcc CAGGTGCAGCTTGTAGAGAC 1 1 --44 TTCACGAGTCCAGCCTCAAGCAGT GAGGTRCAGCTKGTAGAGAC ---VH primer F44 VH-for15 ggcggcggcggctccggtggtggt ggatcc GARGTGMAGCTGKTGGAGAC 8 3 Primers VH-rev 5' 3' outer-rev cggagtcaggcccccgag 5' SfiI VH 3' VH-rev1 cggagtcaggcccccgaggc CGAGGAGACGGTGACMGTGG 2 2 VH-rev2 cggagtcaggcccccgaggc CGCAGAGACAGTGACCAGAG 1 1 ____.SfiI____ (CTCTGGTCACTGTCTCTGCGgcctcgggggcctgactccg) L V T V S A A S G A - VH-rev3 cggagtcaggcccccgaggc CGAGGAGACTGTGAGASTGG 2 2 ========== 2002 Antibody Display Methods and Protocols, Fab, Mouse 3'primer LC Cκ1 CATG TCTAGA ACACTCATTCCTGTTGAAGCTCTTG 5' primers LC VκA CATG GAGCTC GATGTTTTGATGACCCAAACTCCA VκB GATC GAGCTC GACATTGTGCTCACCCAATCTCC VκC CATG GAGCTC GACATTGTGCTRACCCAGTCTTCCA VκD CATG GAGCTC GACATCCAGATGACNCAGTCTCAA VκE CATG GAGCTC CAAATTGTTCTCACCCAGTCTCCA VκF CATG GAGCTC GAAAATGTGCTTCACCCAGTCTCCA 3' primers HC Igγ1 AGGCTT ACTAGT TATGCAAGGCTTACAACC Igγ2A AGGCTT ACTAGT ACAGGGCTTGATTGTGGGCCC Igγ2B AGGCTT ACTAGT ACAGGGGTTCAGTGTTGAAATGG 5' primers HC IA TGGAGGCTT CTCGAG GAKGTGCAGCTTCAGGAGTC IB TGGAGGCTT CTCGAG CAGGTGCAGCTGAAGSAGTC IIA TGGAGGCTT CTCGAG SAGGTCCAGCTGCARCAGTC IIB TGGAGGCTT CTCGAG CAGGTCCARCTGCAGCAGYTTGG IIC TGG AGGCTT CTCGAG GAGGTTCAGCTGCAGCAGTC IIIA TGGAGGCTT CTCGAG GARGTGAAGCTGGTGGARTCTGG IIIB TGGAGGCTT CTCGAG GAGGTGAAGCTTCTGGAGTCTGG IIIC TGGAGGCTT CTCGAG GAAGTGAAGCTTGAGGAGWCTGG IIIDA TGGAGGCTT CTCGAG GAAGTGCAGCTGGTGGAGTCTGG IIIDB TGGAGGCTT CTCGAG GAAGTGATGCTGGTGGAGTCTGG VA TGGAGGCTT CTCGAG GAGGTYCAGCTKCAGCAG CH1 GCCAAAACGACACCCCCA ==== leader sequence for the κ light chains GCCACCATGGAGTTTCAGACCCAGGTACTCATGTCCCTGCTGCTCTGCATGTCTGGTGC M E F Q T Q V L M S L L L C M S G GGCCGCACCAGACATGCAGAGCAGCAGGGACATGAGTACCTGGGTCTGAAACTCCATGGTGGC κ light chains F 3 TCAATAGTTGAACATAGCGGCCGCA SAAAWTGTKCTCACCCAGTC 4 TCAATAGTTGAACATAGCGGCCGCA GAWATTGTGCTMACTCAGTC 5 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTRACACAGTC 6 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGATGACMCAGTC 7 TCAATAGTTGAACATAGCGGCCGCA GAYATCMAGATRAMCCAGTC 8 TCAATAGTTGAACATAGCGGCCGCA GAYATCCAGATGAYTCAGTC 9 TCAATAGTTGAACATAGCGGCCGCA GATATCCAGATGACACAGAC 10 TCAATAGTTGAACATAGCGGCCGCA GACATTGTGCTGACCCAATC 11 TCAATAGTTGAACATAGCGGCCGCA GACATYSTRATGACCCARTC 12 TCAATAGTTGAACATAGCGGCCGCA GATRTTKTGATGACYCARAC 13 TCAATAGTTGAACATAGCGGCCGCA GAYATTGTGATGACBCAGKC 14 TCAATAGTTGAACATAGCGGCCGCA GATATTGTGATAACCCAGGA 15 TCAATAGTTGAACATAGCGGCCGCA GACATCYTGCTGACYCAGTC 16 TCAATAGTTGAACATAGCGGCCGCA GAAAWTGTGYTGACCCAGTC 17 TCAATAGTTGAACATAGCGGCCGCA GAAACAACTGTGACCCAGTC 18 TCAATAGTTGAACATAGCGGCCGCA GACATTRTGATGWCACAGTC 19 TCAATAGTTGAACATAGCGGCCGCA GACATCCAGMTGACMCARTC κ light chains R 20 GGATACAGTTGGTGCAGCATCAGCCC Primers used for the generation of the joining fragment 21 GGGCTGATGCTGCACCAACTGTA 22 TCAAGTGCAAAGACTCACTTTATTG 23 CAATAAAGTGAGTCTTTGCACTTGATAGTTATTAATAGTAATCAATTACG 24 TAGCAGAACAGGCAGCTTCATGGTGGCCTGGGAGTGGACACCTGTGGAGAG 25 ATGAAGCTGCCTGTTCTGCTAGTG 26 ACTGCTTGAGGCTGGACTCGTGAACAATAGCAGC Degenerate forward primers used for the amplification of rearranged heavy chains 27 TTCACGAGTCCAGCCTCAAGCAGT GAKRTRCAGCTTMAGGAGTC 28 TTCACGAGTCCAGCCTCAAGCAGT GAGKTYCAGCTBCAGCAGTC 29 TTCACGAGTCCAGCCTCAAGCAGT CAGGTGCAGMTGAAGSAGTC 30 TTCACGAGTCCAGCCTCAAGCAGT GAGRTCCAGCTGCAACARTC 31 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYVAGCTGCAGCAGTC 32 TTCACGAGTCCAGCCTCAAGCAGT CAGGTYCARCTGCAGCAGTC 33 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGMAGCTGGTGGAATC 34 TTCACGAGTCCAGCCTCAAGCAGT GAVGTGMWGCTSGTGGAGTC 35 TTCACGAGTCCAGCCTCAAGCAGT GARGTGCAGCTGKTGGAGWC 36 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTGATGGAATC 37 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGCAGCTTGTTGAGTC 38 TTCACGAGTCCAGCCTCAAGCAGT GAGGTGAAGCTTCTCRAGTC 39 TTCACGAGTCCAGCCTCAAGCAGT GAAGTGAARMTTGAGGAGTC 40 TTCACGAGTCCAGCCTCAAGCAGT CAGGTTACTCWGAAAGWGTCTG 41 TTCACGAGTCCAGCCTCAAGCAGT CAGGTCCAACTGCAGCAGCC 42 TTCACGAGTCCAGCCTCAAGCAGT GATGTGAACCTGGAAGTGTC 43 TTCACGAGTCCAGCCTCAAGCAGT CAGATCCAGTTSGTRCAGTC 44 TTCACGAGTCCAGCCTCAAGCAGT GAGGTRCAGCTKGTAGAGAC Reverse primers used for the amplification of rearranged heavy chains 45 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACCGTGGTCC (GGACCACGGTCACCGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG) T T V T V S S A R L D - 46 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACTGTGAGAGTGGTGC (GCACCACTCTCACAGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG) T T L T V S S A R L D - 47 CTATTCTAGCTAATCTAGGCGCGCCGCAGAGACAGTGACCAGAGTCC (GGACTCTGGTCACTGTCTCTGCGGCGCGCCTAGATTAGCTAGAATAG) T L V T V S A A R L D - 48 CTATTCTAGCTAATCTAGGCGCGCCGAGGAGACGGTGACTGAGGTTC (GAACCTCAGTCACCGTCTCCTCGGCGCGCCTAGATTAGCTAGAATAG) T S V T V S S A R L D - ====== Evaluation of Antigen-Conjugated Fluorescent Beads to Identify Antigen-Specific B Cells VH1L CCATGGACTGGACCTGGA Heavy chain VH2L CAGATGGACATACTTTGTTCCAC Heavy chain VH3L CCATGGAGTTTGGGCTGAGC Heavy chain VH4L CGATGAAACACCTGTGGTTCTT Heavy chain VH5L ATGGGGTCAACCGCCATCCT Heavy chain VH6L GATGTCTGTCTCCTTCCTCAT Heavy chain VH1F CAGGTGCAGCTGGTGCAGTCTG Heavy chain VH2F GTCTTGTCCCAGGTCAACTTAAGGGAGTCTT Heavy chain VH3F GAGGTGCAGCTGGTGGAGTCTG Heavy chain VH4F CAGGTGCAGCTGCAGGAGTCGG Heavy chain VH5F GAGGTGCAGCTGCTGCAGTCTG Heavy chain VH6F CTGTCACAGGTACAGCTGCAGCAGTCAG Heavy chain IGREV CCAACTCTCTTGTCCACCTTGG Heavy chain L-Vk1/2 ATGAGGGTCCCCGCTCAGCTGCTGG Kappa light chain L-Vk3 CTCTTCCTCCTGCTACTCTGGCTCCCAG Kappa light chain L-Vk4 ATTTCTCTGTTGCTCTGGATCTCTG Kappa light chain Ck 543-566 GTTTCTCGTAGTCTGCTTTGCTCA Kappa light chain Pan-Vk ATGACCCAGACTCCATCCACCCTG Kappa light chain Ck 494-516 GTGCTGTCCTTGCTGTCCTGCT Kappa light chain L-Vλ1 GGTCCTGGGCCCAGTCTGTGCTG Lambda light chain L-Vλ12 GGTCCTGGGCCCAGTCTGCCCTG Lambda light chain L-Vλ3 GCTCTGTGACCTCCTATGAGCTG Lambda light chain L-Vλ4/5 GGTCTCTCTCGCAGCCTGTGCTG Lambda light chain L-Vλ6 GTTCTTGGGCCAATTTTATGCTG Lambda light chain L-Vλ7 GGTCCAATTCCCAGGCTGTGGTG Lambda light chain L-Vλ8 GAGTGGATTCTCAGACTGTGGTG Lambda light chain Cλ-1 CACCAGTGTGGCCTTGTTGGCTTG Lambda light chain F-Vλ1 CAGTCTGTGTTGACGCAGCC Lambda light chain F-Vλ2 CAGTCTGCCCTGACTCAGCC Lambda light chain F-Vλa3 TCTTATGAGCTGACACAGCCA Lambda light chain F-Vλa3l TCTTCTGAGCTGACTCAGGACCC Lambda light chain F-Vλ4ab GAGCTTGTGCTGACTCAATC Lambda light chain F-Vλ4c CTGCCTGTGCTGACTCAGC Lambda light chain F-Vλ5/9 CAGCCTGTGCTGACTCAGCC Lambda light chain F-Vλa6 AATTTTATGCTGACTCAGCCCCACT Lambda light chain F-Vλ7/8 CAGACTGTGGTGACCCAGGAG Lambda light chain F-Vλa10 CAGGCAGGGCTGACTCAG Lambda light chain Cλ-2 GGGTGGGAACAGAGTGACC Lambda light chain ====== HybriFree: a robust and rapid method for the development of monoclonal antibodies from different host species 5'-3' sequences of mouse primers designed by ref. [17] VH forward primer mix proportion in VHF mix Q_mVH_F1 CGTGACCACAGGCGTCCACAGC GAGGTTCDSCTGCAACAGTY 4 Q_mVH_F2 CGTGACCACAGGCGTCCACAGC CAGGTGCAAMTGMAGSAGTC 3 Q_mVH_F3 CGTGACCACAGGCGTCCACAGC GAVGTGMWGCTGGTGGAGTC 4 Q_mVH_F4 CGTGACCACAGGCGTCCACAGC CAGGTTAYTCTGAAAGAGTC 2 Q_mVH_F5 CGTGACCACAGGCGTCCACAGC GAKGTGCAGCTTCAGSAGTC 2 Q_mVH_F6 CGTGACCACAGGCGTCCACAGC GACACAACTGTGACCCAGTC 2 Q_mVH_F7 CGTGACCACAGGCGTCCACAGC CAGRTCCAACTGCAGCAGYC 2 Q_mVH_F8 CGTGACCACAGGCGTCCACAGC GAGGTGMAGCTASTTGAGWC 3 Q_mVH_F9 CGTGACCACAGGCGTCCACAGC GAAGTGAAGMTTGAGGAGTC 2 Q_mVH_F10 CGTGACCACAGGCGTCCACAGC GATGTGAACCTGGAAGTGTC 1 Q_mVH_F11 CGTGACCACAGGCGTCCACAGC CAGATKCAGCTTMAGGAGTC 2 Q_mVH_F12 CGTGACCACAGGCGTCCACAGC CAGGCTTATCTGCAGCAGTC 1 Q_mVH_F13 CGTGACCACAGGCGTCCACAGC CAGGTTCACCTACAACAGTC 1 Q_mVH_F14 CGTGACCACAGGCGTCCACAGC CAGGTGCAGCTTGTAGAGAC 1 Q_mVH_F15 CGTGACCACAGGCGTCCACAGC GARGTGMAGCTGKTGGAGAC 3 VH reverse primer mix proportion in VHR mix Q_mVH_R1 CACCACCACTACCGCCTCCTCCTGAGGAGACGGTGACMGTGG 2 Q_mVH_R2 CACCACCACTACCGCCTCCTCCTGCAGAGACAGTGACCAGAG 1 Q_mVH_R3 CACCACCACTACCGCCTCCTCCTGAGGAGACTGTGAGASTGG 2 VLκ forward primer mix proportion in VLκF mix Q_mVLκ_F1 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACAWTGTTCTCACCCAGTC 2 Q_mVLκ_F2 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATCCAGATGACACAGWC 2 Q_mVLκ_F3 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATRTTGTGATGACCCAGWC 2 Q_mVLκ_F4 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTSTGMTGACCCAGTC 2 Q_mVLκ_F5 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATGTTGTGVTGACCCAAAC 2 Q_mVLκ_F6 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACACAACTGTGACCCAGTC 1 Q_mVLκ_F7 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGAYATTKTGCTCACTCAGTC 2 Q_mVLκ_F8 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATATTGTGATRACCCAGGM 2 Q_mVLκ_F9 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTGTAATGACCCAATC 1 Q_mVLκ_F10 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGACATTGTGATGWCACAGTC 2 Q_mVLκ_F11 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATRTCCAGATGAMCCAGTC 2 Q_mVLκ_F12 GGAGGAGGCGGTAGTGGTGGTGGTGGATCTGGTGGTGGTGGATCCGATGGAGAAACAACACAGGC 1 VLκ reverse primer mix proportion in VLκR mix Q_mVLκ_R1x GGACAGGTGTGGGTCTTGTCAGCCCGTTTBATTTCCAGCTTGG 2 (CCAAGCTGGAAATVAAACGGGCTGACAAGACCCACACCTGTCC) K L E X K R A D K T H T C Q_mVLκ_R2x GGACAGGTGTGGGTCTTGTCAGCCCGTTTTATTTCCAATTTTG 1 (CAAAATTGGAAATAAAACGGGCTGACAAGACCCACACCTGTCC) K L E I K R A D K T H T C </pre> [[Category:Antibodies]]
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