Editing Plasmid Miniprep By Isopropanol and PEG Precipitation

[[File:Plasmid isopropanol ppt.JPG|RTENOTITLE]][[File:PEG ppt Plasmid.JPG|RTENOTITLE]]

[[File:Isopropanol-PEG-Plasmid QC digestion.JPG|RTENOTITLE]]

12/1/2018

Test CaCl2, MgCl2, CTAB for removing genomic DNA and RNA. Add MgCl2, CaCl2 to N3, final conc: 1.5 M in N3

CTAB add 20 ul 5% CTBA to 0.3 ml N3, Add 0.6 ml isopropanol to precipitate DNA . add EB to ethanol washed precipitate

water soluble fraction:



&nbsp;[[File:CATB Ca Mg on Isopropanol Plasmid.JPG|RTENOTITLE]]

Water insoluble fraction, dissolve in 0.4 M NaCl:

http://www.excellgen.net/eln/images/7/74/CATB_Ca_Mg_on_Isopropanol_Plasmid2.JPG

12/2/2018

N3-CaCl2 on removing genomic DNA

[[File:CATB Ca Mg on Isopropanol Plasmid4.JPG|RTENOTITLE]]

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| Sample #
| OD260
| OD260 after PEG-NaCl ppt
|-
| 1,&nbsp;
| 16 ng/ul
| <br/>
|-
| 2
| 2695 ng/ul
| <br/>
|-
| 3
| 35&nbsp;ng/ul
| <br/>
|-
| 4
| 1198&nbsp;&nbsp;ng/ul
| <br/>
|-
| 5
| 2605&nbsp;ng/ul
| 251 ng/ul
|-
| 6<br/>
| 1131&nbsp;ng/ul
| 117 ng/ul
|-
| 7, 2203
| 22 ng/ul
| <br/>
|-
| 8, 2203
| 1494&nbsp;ng/ul
| <br/>
|-
| 9, 2203
| 
| 121&nbsp;ng/ul<br/>
|}
[[Category:Protocols]]