Editing Purify DNA from Gel using Isopropanol

1, Make <1% agarose gel.

2, Run DNA on gel, cut out band into a microcentrifuge tube

3. Add&nbsp; 1~3 vol of QG Buffer.&nbsp;

4. Use warm water bath (60~90 oC) to dissolve gel.

5. Measure final vol using pipette.&nbsp;

6. Add 0.6 X isopropanol. Mix

7. Spin at max speed for 5 min.

8. Dump off liquid.

9. Add 100 ul 70% ethanol.&nbsp;

10. Spin 1 min.

11. Dump off ethanol.

12. Spin 5 seconds.

13. Remove liquid using 10 ul pippett.

14. Dry at 50 oC on heat block for 1 min.

15. Add 5~20 ul ddH2O. Votex.

16.&nbsp; Continue&nbsp;to digestion or ligation.&nbsp;

[[File:DNA recovery isopropanol precipitation.JPG|RTENOTITLE]]

Lane 1: Recover DNA from gel by centrifugation&nbsp;for 15 min using a spin column, then precipitate using isopropanol

Lane 2:&nbsp;Recover DNA from gel by dissolving gel in QG buffer then then precipitate DNA with 0.6 X isopropanol
[[Category:Protocols]]