Editing SOC Medium

'''SOC Medium'''

0.5% yeast extract<br/>2% tryptone<br/>10 mM NaCl<br/>2.5 mM KCl<br/>10 mM MgCl2<br/>10 mM MgSO4<br/>and 20 mM glucose.
<pre>1.Add the following to 90ml of distilled H2O
	2g Bacto Tryptone
	0.5 g Bacto Yeast Extract
	0.25 ml of 4M NaCl.
	0.25 ml of 1M KCl.
	1 ml of 1M MgCl2
	1 ml of 1M MgSO4, optional
	2 ml of 1M glucose


2. Adjust to 100mL with distilled H2O

3. Sterilize by autoclaving
</pre>

or

500 ml '''SOC Medium'''<br/>● 1.802g glucose<br/>● 10g tryptone<br/>● 2.5g Yeast extract<br/>● 0.584g NaCl<br/>● 0.093g KCl<br/>● 1.016g MgCl2 (anhydrous)<br/>○ Note: If you don’t have MgCl2 (anhydrous), use 2.170g of MgCl26H20<br/>● 1.234g MgSO4.7H2O<br/>● 500ml of dH20 (distilled water)



'''SOB Medium'''<br/>0.5% (w/v) yeast extract<br/>2% (w/v) tryptone<br/>10 mM NaCl<br/>2.5 mM KCl<br/>20 mM MgSO4

Per liter:

5 g yeast extract<br/>20 g tryptone<br/>0.584 g NaCl<br/>0.186 g KCl<br/>2.4 g MgSO4<br/>Note: Some formulations of SOB use 10 mM MgCl2 and 10 mM MgSO4 instead of 20 mM MgSO4.

SOB medium is also available dry premixed from Difco, 0443-17.

Adjust to pH 7.5 prior to use. This requires approximately 25 ml of 1M NaOH per liter.
[[Category:Buffer Recipe]]