Editing Signal peptide comparison

Transfection: <br> 

5 ul&nbsp; (100 ~ 200 ng/ul) from miniprep, each well is from individuale clone<br> 

24 well plate.<br> 

1393: pCMV5-IL2-TagGFP-(3c)-Fc-WPRE-OriP<br> 

1394: pCMV5-CST4-TagGFP-Fc(3c)-WPRE-OriP<br> 

1395: pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP <br> 

1396: pCMV5-hIgG-Hc-TagGFP-Fc(3c)-WPRE-OriP<br> 

[[Image:GFP-comparison 2012 10 18.jpg]] 

Nov 2012 

Transfect with L2K. 1433 plasmid DNA quality is low, only 20 ng/ul<br>[[Image:Secretion signal comparison 2012 11 20.jpg]]<br> 

<br> 

[[Image:Signal peptide comp 2012 11 22.jpg]] 

<br> 

2012/11/23 

[[Image:Signal peptides 2012 11 23 1.jpg]] 

11/24/2012 

[[Image:Signal peptides 2012 11 24 1.jpg]] 

[[Image:Signal peptides 2012 11 25.jpg]] 

11/20/2012: Transfection using lipofectamine 2000, 1 ug DNA, 3 ul L2K, 12 well plate<br> 

Picture on 2012/11/22<br> 

[[Image:Signal peptide L2K 2012 11 22.jpg]]<br> 

Picture on 2012/11/23<br> 

[[Image:Signal peptides 2012 11 23 2.jpg]] 

11/24/2012 

[[Image:Signal peptides 2012 11 24 2 1.jpg]] 

2012/11/25 

[[Image:Signal peptides 2012 11 25 2.jpg]][http://www.excellgen.net/eln/images/4/4e/Signal_seq_comparison_2013_1_24.jpg Signal_seq_comparison_2013_1_24.jpg]‎ 

<br> 

11/21/2012: Transfection using GeneExpression Max<br> 

1 ug DNA, 3 ul GEM, 12 well plate<br> 

Picture on 11/22/2012 

[[Image:Signal peptides GEM 2012 11 12.jpg]] 

<br> 

[[Image:Signal peptides 2012 11 23 3.jpg]] 

2012/11/24 

[[Image:Signal peptides 2012 11 24 3 2.jpg]] 

2012/11/25 

[[Image:Signal peptide comp 2012 11 25.jpg]] 

2012/12/12 

transfection on 2012/12/11: 1 ug/well, 3 ul GEM, picture taken on 2012/12/12 

<br> 

[[Image:Signal peptide comparison 2012 12 12.jpg]] 

2012/12/13 

[[Image:Signal peptide comparison 2012 12 13.jpg]] 

2012/12/14 

[[Image:Signal peptide comparison 2012 12 14.jpg]] 

plate 

[[Image:Signal peptide comparison plate 2012 12 14.jpg]] 

= Compare new IL2 signal peptide vs other  =

12 well plate, 293T cells 

Transfection: 1 ug DNA 

Transfection reagent: GEM 

Date: 2013/1/24 

<br> 

2013 1 25 

[[Image:Signal seq comparison 2013 1 24.jpg]] 

= [[Image:Signal seq comparison 2013 1 25.jpg]]<br>  =

2013/1/26

[[Image:Signal_seq_comparison_2013_1_26.JPG]]

[[Category:QC]] [[Category:293_Expression]]