Editing Synthesis Lipopolymer

Synthesis of Lipopolymer by N-acylation of 2 kDa PEI with lipids chlorides. 

1. Dissolve 100 mg PEI (2 kDa) in 5 mL of DCM Dichloromethane under N2 at room temperature. <br>2. Add 160 ÎŒL TEA Triethylamine.<br>3. Dissolve fatty acid in 5 mL of DCM.<br>4. Slowly add lipid to the PEI solution over a 30 min period. <br>5. Stir for 12 hrs under N2.<br>6. Stop the reaction by placing solution in ice bath.<br>7. Concentrate reaction mixture in vacuo<br>8. Wash 3 times with excess of ethyl ether. <br>9. Dry residue overnight under vacuum at room temperature.<br>C8: Caprylic Acid<br>C14: Myristic Acid<br>C16: Palmitic Acid<br>C18: Stearic Acid<br>C18:1: Oleic Acid<br>C18:2: Linoleic Acid<br> 

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'''Synthesis Lipid-Substituted Polymers by''' '''N-acylation of PLL with activated fatty acid NHS-esters'''<br>

<br>Syntheis of NHS esters of fatty acids (NHS-X, were X= C8, C14, C16, C18, C18:1, C8:2)<br>

1. Mix equal molar amounts of fatty acid, NHS, and DCC in dry ethyl acetate (12 mL).<br>

2. Stir the reaction mixture overnight at room temperature under argon atmosphere. <br>

3. Remove solid dicyclohexylurea by-product formed by filtration.<br>

4. Concentrate the filtrate under reduced pressure. <br>

5. Recrystallize the crude NHS-X in ethanol at 4 oC<br>

<br>1. Dissolve NHS-X in 1 mL of DMF and add dropwise to 15 mg of PLL in 1 mL of DMSO. (the fatty acid:PLL amine ratio is either 1:1.5 or 1:6).<br>

2. Let react&nbsp; 24 h at room temperature under argon.<br>

3. Add excess ethyl ether to&nbsp; precipitate&nbsp; the polymers,&nbsp;and then wash 3 times with ethyl ether.

4. Dry the polymers&nbsp; under vacuum at ambient temperature overnight.<br><br>

[[Category:Transfection]] [[Category:Paul]]