Editing Synthesis Thiolated Chitosan

Chitosan-cysteine<br>conjugates synthesized by the addition of 50 mM EDAC has the highest content of thiol<br>groups of 265,169 μmol per 50 mg of conjugates.<br>

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Synthesis of chitosan-cysteine conjugates<br>Chitosan-cysteine conjugates are made by: prepared beaker glass capacity 500 mL of<br>4 pieces. Made of 1% acetic acid solution of 250 mL in each beaker. Then into each beaker<br>glass was dissolved 5 g of chitosan. After all the soluble chitosan, into each beaker glass is<br>added 2,5 g of cysteine until dissolved. Then added with a concentration 0 mM EDAC, 30<br>mM, 40 mM, and 50 mM in each solution. pH value adjusted to 5 with the addition of NaOH<br>1 N. The mixture was incubated for 3 hours with continous stirring. The solution was<br>dialyzed twice in a dialysis tubing (MW cut-off 12-14 kDa) protected from light at 100C with<br>1 mM HCl containing 1% NaCl for 1 day and then with 1 mM HCl for 2 days to eliminate<br>EDAC and cysteine of the conjugate solution. The solution is lyophilized and conjugates (dry<br>conditions) maintained at 4oC until ready for use (Roldo et al., 2004).<br>

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