Editing Yijia LI 5 14 2018

'''Miniprep digestion of 3122_984_3192_3194'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| Lane
| Enzymes
| Size
| Note
|-
| 3122
| 1-4
| Nde1+Xho1
| <br/>
| <br/>
|-
| 984
| 5-8
| Xba1+Nhe1
| 957bp
| Lane 8 maybe good,need to confirm the size
|-
| 3192
| 9-12
| Kpn1
| <br/>
| need to confirm the size
|-
| 3194
| 13-16
| Nhe1+Kpn1
| <br/>
| Maybe vector
|}

[[File:Miniprep dogestion of 3122 984 3192 3194.JPG|RTENOTITLE]]

'''Miniprep digestion of 1000_3200_3166'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| Lane
| Enzymes
| Size
| Note
|-
| 1000
| 1-3
| HindIII+Xho1
| 100
| need to confirm the size
|-
| 3200
| 4-6
| BamH1+ECoR1
| <br/>
| no insert band should be good,redo
|-
| 3166
| 7-10
| Sal1+BsrG1<br/>
| 1234
| need to confirm the size
|}

[[File:Miniprep digestion of 1000 3200 3166.JPG|RTENOTITLE]]

'''Miniprep digestion of 3191_931_3197'''

{| border="1" cellspacing="1" cellpadding="1" style="width:500px;"
|-
| #
| Lane
| ENzymes
| Size
| Note
|-
| 3191
| 1
| <br/>
| <br/>
| Not good, redo
|-
| 931
| 2,3
| 2--HindIII+Nde1;3--HindIII+Xho1
| both are around 900 bp
| seems good, but lysis
|-
| 3197
| 4,5
| <br/>
| <br/>
| not good, lysis
|}

[[File:Miniprep digestin3191 931 3197.JPG|RTENOTITLE]]

== Conclusion ==

Potentially Good:984,3192,3166
[[Category:My ELN]]