GuideRNA: Revision history

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14 May 2013

  • curprev 13:5113:51, 14 May 2013 Paul.xu talk contribs 1,225 bytes +1,225 Created page with "1. Annealing Buffer<br> *a. 400 μl 1M Tris @ pH 8 *b. 200 μl MgCl2 *c. 100 μl 5M NaCl *d. 20 μl 0.5M EDTA @ pH8 *e. 280 μl H2O 2. BsaI-HF<br>3. NEB 4<br>4. Ligase<br>5. QL..."