Pfusion DNA Polymerase Purification: Revision history

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11 September 2013

  • curprev 12:4012:40, 11 September 2013 Paul.xu talk contribs 1,965 bytes +1,965 Created page with "Suspend 183 g of cells in 550 ml of buffer A (10 mM KPO4 buffer, pH 7.4; 1.0 mM EDTA, 1.0 mM beta-mercaptoethanol) containing 0.1 M NaCl Sonicate for 5 minutes at 4° C. Spin t..."
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