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This special page shows all uploaded files.
| Date | Name | Thumbnail | Size | User | Description | Versions |
|---|---|---|---|---|---|---|
| 15:13, 25 October 2010 | IMG 0157.JPG (file) |  |
107 KB | Crm (talk | contribs) | plasmid DNA vp16-pET28b digest with Nco1+ Xho1 | 1 |
| 15:14, 25 October 2010 | IMG 0156.JPG (file) |  |
132 KB | Crm (talk | contribs) | plasmid DNA Vp16-pET28b digest with Nde1+ Xho1, using Fermentas enzyme | 1 |
| 17:30, 25 October 2010 | IMG 0158.JPG (file) |  |
86 KB | Paul.xu (talk | contribs) | Nde1+ Xho1 cut, NEB enzymes | 1 |
| 18:56, 25 October 2010 | IMG 0159.JPG (file) |  |
98 KB | Paul.xu (talk | contribs) | Digest sample 1, 2 and 8 with Nde1, Xho1 (Fermantas) and AseI (NEB) using Fermentas buffer. | 1 |
| 22:47, 25 October 2010 | PVp16-1.txt (file) |  |
39 KB | Paul.xu (talk | contribs) | Vp16 gene synthesis design | 1 |
| 23:05, 25 October 2010 | VP16 Xho Xba.JPG (file) |  |
114 KB | Paul.xu (talk | contribs) | Vp16-pET28b digested with Xho1, or Xho1+Xba1, or undigested | 1 |
| 12:07, 26 October 2010 | Vp16-pET28b PCR Screening.jpg (file) |  |
125 KB | Crm (talk | contribs) | VP16 colony screening using PCR, T7F + Vp16-36R primers | 1 |
| 17:40, 26 October 2010 | VP16 pET28b PCR Screening 2.jpg (file) |  |
191 KB | Paul.xu (talk | contribs) | PCR screening of VP16-pET28b colonies | 1 |
| 17:53, 26 October 2010 | Vp16-pET28b PCR Screening 2.jpg (file) |  |
109 KB | Paul.xu (talk | contribs) | VP16-pET28b PCR Screening 2nd exp, 2nd gel | 1 |
| 13:14, 27 October 2010 | Vp16 pET28 Nde1 Xho1.jpg (file) |  |
138 KB | Paul.xu (talk | contribs) | Vp16-pET28b Nde1+Xho1 | 1 |
| 12:40, 29 October 2010 | 10 28 2010 VP16.tar.gz.txt (file) | |
854 KB | Paul.xu (talk | contribs) | VP16-pET28b sequencing result | 1 |
| 15:42, 31 October 2010 | VP16 pET28b pDBHisMBP Nde1 Xho1.JPG (file) |  |
60 KB | Paul.xu (talk | contribs) | Vp16-pET28b, VP16-pDB.His.MBP digest with Nde1+Xho1 | 1 |
| 16:57, 31 October 2010 | VP16 pET28b pDBHisMBP Nco Xho1.JPG (file) |  |
66 KB | Paul.xu (talk | contribs) | VP16-pET28b, VP16-pDBHisMBP, digest with Nco1, Xho1 | 1 |
| 19:53, 31 October 2010 | VP16 pET28b pDBHisMBP Nde1 Xho1 NEB.JPG (file) |  |
79 KB | Paul.xu (talk | contribs) | VP16-pET28b,VP16-pDBHisMBP digest with Nde+Xho1 (NEB enzyme) | 1 |
| 19:54, 31 October 2010 | VP16 pET28b Clone24 Nde1 Xho1 NEB Fastdigest.JPG (file) |  |
63 KB | Paul.xu (talk | contribs) | VP16-pET28b (Clone24) digest with Nde1+Xho1 (Fastdigest left, NEB right) | 1 |
| 16:21, 5 November 2010 | Aldolase Vp16 MBP fusion expression.jpg (file) | Error creating thumbnail: File with dimensions greater than 12.5 MP |
4.33 MB | Paul.xu (talk | contribs) | expression of Aldolase-MBP and VP16-MBP fusion proteins in BL21(DE3). | 1 |
| 16:34, 5 November 2010 | Vp16-MBP expression.jpg (file) |  |
132 KB | Paul.xu (talk | contribs) | VP16-MBP Expression in BL21 cells | 1 |
| 17:39, 5 November 2010 | POTB7-PEDF-R-EcoR1 Xho1.JPG (file) |  |
40 KB | Paul.xu (talk | contribs) | pOTB7-PEDFR cut with EcoR1 & Xho1, NM_020376, size should be 1.5 kb, cut only ~600 bp | 1 |
| 18:17, 8 November 2010 | VP16 pDB His GST pDB His NusA Nde1 Xho1.JPG (file) |  |
78 KB | Paul.xu (talk | contribs) | pDB.His.GST-Vp16 & pDB.His.NusA, digest with Nde1 + Xho1 | 1 |
| 12:52, 13 November 2010 | Vp16 NusA MBP GST expression.jpg (file) |  |
135 KB | Paul.xu (talk | contribs) | expression of VP16 fusion to NusA, MBP, GST | 1 |
| 14:50, 14 November 2010 | PLenti-HA AKR1C4 BamH1 Xho1.jpg (file) |  |
48 KB | Paul.xu (talk | contribs) | pLenti-HA-AKR1C4 digest with BamH1 + Xho1 | 1 |
| 19:41, 19 November 2010 | NusA VP16 TEV Digestion 2.jpg (file) |  |
29 KB | Paul.xu (talk | contribs) | NusA-VP16 fusion protein digested with TEV | 1 |
| 19:48, 19 November 2010 | NusA VP16 TEV Digestion.jpg (file) |  |
86 KB | Paul.xu (talk | contribs) | NusA_VP16_TEV_Digestion | 1 |
| 13:34, 12 December 2010 | VP16 purification WB.jpg (file) |  |
130 KB | Paul.xu (talk | contribs) | 6X-His-VP16 expression and purification | 1 |
| 19:39, 11 January 2011 | Lv mastermix optimazation.JPG (file) |  |
183 KB | Paul.xu (talk | contribs) | Lv mastermix optimization and comparison of transfection reagents | 1 |
| 17:42, 26 January 2011 | Lentivirus vector transfection optimization.JPG (file) |  |
187 KB | Paul.xu (talk | contribs) | 1 | |
| 09:36, 27 January 2011 | Lentivirus packaging mix optimization day 2.JPG (file) |  |
207 KB | Paul.xu (talk | contribs) | 1 | |
| 17:26, 28 January 2011 | Lentivirus packaging mix optimizationg 2 day1.JPG (file) |  |
212 KB | Paul.xu (talk | contribs) | 1 | |
| 18:28, 28 January 2011 | Vp16 MBP purification.jpg (file) |  |
77 KB | Paul.xu (talk | contribs) | 1 | |
| 13:47, 29 January 2011 | Packagingmix optimization 2 day2.JPG (file) |  |
203 KB | Paul.xu (talk | contribs) | 1 | |
| 13:52, 29 January 2011 | Packagingmix optimization 1 infection day1.JPG (file) |  |
234 KB | Paul.xu (talk | contribs) | 1 | |
| 11:42, 30 January 2011 | Packagingmix optimization 3 day2 after transfection.JPG (file) |  |
222 KB | Paul.xu (talk | contribs) | 1 | |
| 11:42, 30 January 2011 | Packagingmix optimization 2 day1 post infection.JPG (file) |  |
205 KB | Paul.xu (talk | contribs) | 1 | |
| 11:42, 30 January 2011 | Packagingmix optimization 1 day3 post infection.JPG (file) |  |
204 KB | Paul.xu (talk | contribs) | 1 | |
| 15:47, 30 January 2011 | Lentivirus packaging mix optimizationg 3 day2.JPG (file) |  |
216 KB | Paul.xu (talk | contribs) | 1 | |
| 18:25, 31 January 2011 | Mbp vp16 purification.jpg (file) |  |
89 KB | Paul.xu (talk | contribs) | 1 | |
| 18:25, 31 January 2011 | MBP vp16 purification2.jpg (file) |  |
77 KB | Paul.xu (talk | contribs) | 1 | |
| 12:11, 1 February 2011 | Lentivirus packaging mix optimizationg 1 infection day3.JPG (file) |  |
206 KB | Paul.xu (talk | contribs) | 1 | |
| 15:07, 1 February 2011 | Lentivirus packaging mix optimization 2 infection day2.JPG (file) |  |
205 KB | Paul.xu (talk | contribs) | 1 | |
| 15:12, 1 February 2011 | Lentivirus packaging mix optimization 3 days 3.JPG (file) |  |
217 KB | Paul.xu (talk | contribs) | 1 | |
| 15:13, 1 February 2011 | Lentivirus packaging mix optimization 3 infection day1.JPG (file) |  |
206 KB | Paul.xu (talk | contribs) | 1 | |
| 11:07, 3 February 2011 | Lentivirus packaging mix optimization 3 day4 .JPG (file) |  |
227 KB | Ztao (talk | contribs) | 1 | |
| 11:12, 3 February 2011 | Lentivirus packaging mix 2optimization 3 two days after infection.JPG (file) |  |
182 KB | Ztao (talk | contribs) | 1 | |
| 11:33, 3 February 2011 | Lentivirus packaging mix optimization 2 infection day3.JPG (file) |  |
212 KB | Paul.xu (talk | contribs) | 1 | |
| 12:04, 3 February 2011 | Lentivirus packaging mix PNPLA2 367881 BPEI25 day 1 after transfection.JPG (file) |  |
285 KB | Ztao (talk | contribs) | 1 | |
| 10:32, 4 February 2011 | Transfection reagent comparison 1 1 day after transfection.JPG (file) |  |
206 KB | Ztao (talk | contribs) | 1 | |
| 10:36, 4 February 2011 | Lentivirus packaging mix optimization 3 three days after infection.JPG (file) |  |
206 KB | Paul.xu (talk | contribs) | 1 | |
| 10:37, 4 February 2011 | Lentivirus packaging mix optimization 1 day 6 post infection.JPG (file) |  |
204 KB | Paul.xu (talk | contribs) | 1 | |
| 10:37, 4 February 2011 | Lentivirus packaging mix optimization 1 day 5 post infection.JPG (file) |  |
212 KB | Paul.xu (talk | contribs) | 2 | |
| 10:44, 4 February 2011 | Lentivirus packaging mix optimization 3 day5.JPG (file) |  |
214 KB | Paul.xu (talk | contribs) | 1 |
