Uploads by Paul.xu

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Date Name Thumbnail Size Description Versions
17:30, 25 October 2010 IMG 0158.JPG (file) 86 KB Nde1+ Xho1 cut, NEB enzymes 1
18:56, 25 October 2010 IMG 0159.JPG (file) 98 KB Digest sample 1, 2 and 8 with Nde1, Xho1 (Fermantas) and AseI (NEB) using Fermentas buffer. 1
22:47, 25 October 2010 PVp16-1.txt (file) 39 KB Vp16 gene synthesis design 1
23:05, 25 October 2010 VP16 Xho Xba.JPG (file) 114 KB Vp16-pET28b digested with Xho1, or Xho1+Xba1, or undigested 1
17:40, 26 October 2010 VP16 pET28b PCR Screening 2.jpg (file) 191 KB PCR screening of VP16-pET28b colonies 1
17:53, 26 October 2010 Vp16-pET28b PCR Screening 2.jpg (file) 109 KB VP16-pET28b PCR Screening 2nd exp, 2nd gel 1
13:14, 27 October 2010 Vp16 pET28 Nde1 Xho1.jpg (file) 138 KB Vp16-pET28b Nde1+Xho1 1
12:40, 29 October 2010 10 28 2010 VP16.tar.gz.txt (file) 854 KB VP16-pET28b sequencing result 1
15:42, 31 October 2010 VP16 pET28b pDBHisMBP Nde1 Xho1.JPG (file) 60 KB Vp16-pET28b, VP16-pDB.His.MBP digest with Nde1+Xho1 1
16:57, 31 October 2010 VP16 pET28b pDBHisMBP Nco Xho1.JPG (file) 66 KB VP16-pET28b, VP16-pDBHisMBP, digest with Nco1, Xho1 1
19:53, 31 October 2010 VP16 pET28b pDBHisMBP Nde1 Xho1 NEB.JPG (file) 79 KB VP16-pET28b,VP16-pDBHisMBP digest with Nde+Xho1 (NEB enzyme) 1
19:54, 31 October 2010 VP16 pET28b Clone24 Nde1 Xho1 NEB Fastdigest.JPG (file) 63 KB VP16-pET28b (Clone24) digest with Nde1+Xho1 (Fastdigest left, NEB right) 1
16:21, 5 November 2010 Aldolase Vp16 MBP fusion expression.jpg (file)
Error creating thumbnail: File with dimensions greater than 12.5 MP
4.33 MB expression of Aldolase-MBP and VP16-MBP fusion proteins in BL21(DE3). 1
16:34, 5 November 2010 Vp16-MBP expression.jpg (file) 132 KB VP16-MBP Expression in BL21 cells 1
17:39, 5 November 2010 POTB7-PEDF-R-EcoR1 Xho1.JPG (file) 40 KB pOTB7-PEDFR cut with EcoR1 & Xho1, NM_020376, size should be 1.5 kb, cut only ~600 bp 1
18:17, 8 November 2010 VP16 pDB His GST pDB His NusA Nde1 Xho1.JPG (file) 78 KB pDB.His.GST-Vp16 & pDB.His.NusA, digest with Nde1 + Xho1 1
12:52, 13 November 2010 Vp16 NusA MBP GST expression.jpg (file) 135 KB expression of VP16 fusion to NusA, MBP, GST 1
14:50, 14 November 2010 PLenti-HA AKR1C4 BamH1 Xho1.jpg (file) 48 KB pLenti-HA-AKR1C4 digest with BamH1 + Xho1 1
19:41, 19 November 2010 NusA VP16 TEV Digestion 2.jpg (file) 29 KB NusA-VP16 fusion protein digested with TEV 1
19:48, 19 November 2010 NusA VP16 TEV Digestion.jpg (file) 86 KB NusA_VP16_TEV_Digestion 1
13:34, 12 December 2010 VP16 purification WB.jpg (file) 130 KB 6X-His-VP16 expression and purification 1
19:39, 11 January 2011 Lv mastermix optimazation.JPG (file) 183 KB Lv mastermix optimization and comparison of transfection reagents 1
17:42, 26 January 2011 Lentivirus vector transfection optimization.JPG (file) 187 KB   1
09:36, 27 January 2011 Lentivirus packaging mix optimization day 2.JPG (file) 207 KB   1
17:26, 28 January 2011 Lentivirus packaging mix optimizationg 2 day1.JPG (file) 212 KB   1
18:28, 28 January 2011 Vp16 MBP purification.jpg (file) 77 KB   1
13:47, 29 January 2011 Packagingmix optimization 2 day2.JPG (file) 203 KB   1
13:52, 29 January 2011 Packagingmix optimization 1 infection day1.JPG (file) 234 KB   1
11:42, 30 January 2011 Packagingmix optimization 3 day2 after transfection.JPG (file) 222 KB   1
11:42, 30 January 2011 Packagingmix optimization 2 day1 post infection.JPG (file) 205 KB   1
11:42, 30 January 2011 Packagingmix optimization 1 day3 post infection.JPG (file) 204 KB   1
15:47, 30 January 2011 Lentivirus packaging mix optimizationg 3 day2.JPG (file) 216 KB   1
18:25, 31 January 2011 Mbp vp16 purification.jpg (file) 89 KB   1
18:25, 31 January 2011 MBP vp16 purification2.jpg (file) 77 KB   1
12:11, 1 February 2011 Lentivirus packaging mix optimizationg 1 infection day3.JPG (file) 206 KB   1
15:07, 1 February 2011 Lentivirus packaging mix optimization 2 infection day2.JPG (file) 205 KB   1
15:12, 1 February 2011 Lentivirus packaging mix optimization 3 days 3.JPG (file) 217 KB   1
15:13, 1 February 2011 Lentivirus packaging mix optimization 3 infection day1.JPG (file) 206 KB   1
11:33, 3 February 2011 Lentivirus packaging mix optimization 2 infection day3.JPG (file) 212 KB   1
10:36, 4 February 2011 Lentivirus packaging mix optimization 3 three days after infection.JPG (file) 206 KB   1
10:37, 4 February 2011 Lentivirus packaging mix optimization 1 day 6 post infection.JPG (file) 204 KB   1
10:37, 4 February 2011 Lentivirus packaging mix optimization 1 day 5 post infection.JPG (file) 212 KB   2
10:44, 4 February 2011 Lentivirus packaging mix optimization 3 day5.JPG (file) 214 KB   1
15:11, 5 February 2011 Transfection reagent comparison 2 one day after transfection.JPG (file) 210 KB   1
15:11, 5 February 2011 Transfection reagent comparison 1 two days after transfection.JPG (file) 199 KB   1
15:21, 5 February 2011 Lentivirus packaging mix optimization 2 infection day5.JPG (file) 199 KB   1
15:21, 5 February 2011 Lentivirus packaging mix optimization 2 infection day4.JPG (file) 206 KB   2
15:32, 5 February 2011 Lentivirus packaging mix optimization 3 four days after infection.JPG (file) 197 KB   1
16:55, 5 February 2011 Transfection reagent comparison 2 one day after transfection2.JPG (file) 204 KB   1
11:30, 6 February 2011 Lentivirus packagingmix optimization 3 five days after infection.JPG (file) 207 KB   1
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