1885 pT7A3-HC: Difference between revisions
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Latest revision as of 16:18, 25 October 2013
Cloning of 1885 pT7A3-HC
[edit]Purpose: Cloning of 1885 pT7A3-HC
Date: 2013-10-21---
Notebook:0
Page:
Clone ID:1885
Experimenters: shanyi
Results
[edit]CLONING
[edit]digest pRsetA with DraIII + Sap1---> 2145 bp fragment (ampR + pUC orig), top band
Digest pT7A3 with DraIII + Sap1-->3412 bp fragment (promoter, MCS, LacI), top band
Colony Screening
[edit]Date: 2013-10-23
grow on Amp, but won't grow on Kan,
Make miniprep, digest with EcoRV + EcoR1 should have 2 fragments: the small one should be ~ 1381 bp fragment
STOCK1885-1