2019-pT7A3-Endo-III: Difference between revisions
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Latest revision as of 11:32, 4 February 2014
Cloning of 2019-pT7A3-Endo-III
[edit]Purpose: Cloning of 2019-pT7A3-Endo-III
Date: 2014-1-16--2014-1-24
Notebook:0
Page:
Clone ID:2019
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr from bl21(de3)
ecoli-Endo-III-Nde1-F
ecoli-Endo-III-Xho1-R
| lane |
9 |
10 |
| pcr |
1885 pT7A3-HC | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-1-24
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]
Subcloning of 2019-pT7A3E-Endo-III
[edit]Purpose: Subcloning of 2019-pT7A3E-Endo-III
Date: 2014-1-28------2014-1-31
Notebook:0
Page:
Clone ID:2019
Experimenters: yingying
Results
[edit]cloning
[edit]
| LANE |
3 |
4 |
| 2019-1 |
1892 pT7A3E | |
| Nde1 + Xho1 | ||
Colony Screening
[edit]Date: 2014-1-31
Make miniprep and digest with Nde1 + Xho1
