2048-pEB-C6H: Difference between revisions
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Latest revision as of 09:30, 12 March 2014
Cloning of 2048-pEB-C6H
[edit]Purpose: Cloning of 2048-pEB-C6H
Date: 2014-3-5---2014-3-7
Notebook:0
Page:
Clone ID:2048
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr1:pBadE-1 to pBadE-4
pcr2:PCR fragment from the vector using primers
pBad-Age1-F
pBad-R1
PCR:pBadE-4 +pBad-Age1-F
| lane |
3 |
4 |
| pcr |
545 pBad24 | |
| Agei + HindIII | ||
Colony Screening
[edit]Date: 2014-3-7
Make miniprep and digest with Xho1 + EcoRV
