2098 pFastBac-Dual-VSVG-RSVGag-FLP: Difference between revisions

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Latest revision as of 12:30, 18 March 2014

Cloning of 2098 pFastBac-Dual-VSVG-RSVGag-FLP

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Purpose: Cloning of 2098 pFastBac-Dual-VSVG-RSVGag-FLP==

Date: 2014-3----

Notebook:0

Page:

Clone ID:2098

Experimenters:  shanyi

Results

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CLONING

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subclone from 2076 pCMV5-RSV-Gag-NLS-Flp-WPRE-oriP using BamH1 + XbaI to 2100 pFastBac-Dual-VSVG



Colony Screening

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Date: 2014

Make miniprep, digest with BamH1 + XbaI


digest with BamHI+EcoRI+XbaI

DIGEST WITH KpnI,WILL GET THREE BANDS





Result intepretation/Summary

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Conclusion

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