1078 1203 1245 1026 1557 1802 1819 1833 1886 1887 1898 1929 1930 1935 1937 1938 1939 1943 2025 2026 2194 2196

Protein Expression

2014/5/22
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pET28b-scIHF2	scIHF2	1078	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	22.76	no
2	pET42b-scIHF2	scIHF2	1203	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	22.76	no
3	pDB.His.PfuMBP.scIHF2	scIHF2	1245	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	S		no
4	pET28b-LambdaExo	LambdaExo	1026	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	6.26/28.1
5	pT7A3-MBP-(3C)-Lambda-Exo	MBP-(3C)-Lambda-Exo	1557	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.32/70.5	only Lambda-Exo
6	pageRuler Plus Prestained protein ladder
7	pT7A3-Tth-Ligase-K294R	Tth-Ligase-K294R	1802	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	6.56/79.1	no
8	pT7A30-TthDNALigase-K294R-C-6XHis	TthDNALigase-K294R-C-6XHis	1819	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	6.43/78.5	no
9	pT7A3-TRX	thioredoxin	1833	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.21/17.2	yes
10	pT7A3-T7-DNA-Polymerase	T7-DNA-Polymerase	1886	15 ml, Tuner(DE3)plysN	0.3mM IPTG RT O/N	S	6.24/81.9	no
11	pT7A3-T7-DNA-Pol-D28	T7-DNA-Pol-D28	1887	15 ml, Tuner(DE3)plysN	0.3mM IPTG RT O/N	S	6.1/78.9	no
12	pT7A3-8H-Bst-Exo-	8H-Bst-Exo-	1898	15 ml, Nico21(DE3)plysN		s	5.86/69	yes

Lane	Name	Protein Name	Clone ID	Host	Induction	SAMPLE	pI/Mw	express
1	pT7A30-Ecoli-Ligase	Ecoli-Ligase	1929	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.58/75.3	YES
2	pT7A3-T3-DNA-Ligase-sso7d-6H	-DNA-Ligase-sso7d-6H	1930	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.84/48.3	no
3	pT7A3E-Bst1-Exo-HI	-Bst1-Exo-HI	1935	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	S	5.81/81.2	yes
4	pT7A3E-DNA-Pol-1	DNA-Pol-1	1937	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.55/105.5	yes
5	pT7A3E-Klenow(3'->5'_exo–)	Klenow(3'->5'_exo–)	1938	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.95/70.5	yes
6	pT7A3E-Klenow	Klenow	1939	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	5.84/70.6	yes
7	pT7A3E-T7-RNAPol-Y639F/H784A	T7-RNAPol-Y639F/H784A	1943	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	6.75/100.6	yes
8	pT7A3E-T4-Ligase-HNH-6H	T4-Ligase-HNH-6H	2025	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	6.5/61.7	yes
9	pT7A3E-T4-Ligase-HI-6H	T4-Ligase-HI-6H	2026	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	s	6.39/69	yes
10	pageRuler Plus Prestained protein ladder
11	pT7A3E-HC-Tth-End-IV	-HC-Tth-End-IV	2194	15 ml, Nico21(DE3)plysN	0.3mM IPTG RT O/N	S	6.16/30.6	yes
12	pT7A3E-PBCV-1-Lig-	PBCV-1-Lig	2196	15 ml, Nico21(DE3)plysN		s	6.68/34.2	yes