Cre VLP Activity assay 20140509: Difference between revisions

From ELN
Jump to navigation Jump to search
Paul.xu (talk | contribs)
confirmed, paul, Administrators
13,447 edits
No edit summary
 
(No difference)

Latest revision as of 10:30, 13 June 2014


= Making VLP
= 2123 pCMV5-Mfge8-Tat-Cre-MFGE8 + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2119 pCMV5-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2138 pCMV5-pene-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2136 pCMV5-tat-CD63-iCre + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP +/-VSVG
2172 pCMV5-CD16-Tat-iCre-RIgE + /- 1968 pCMV5-Gag-p2b-NLS-Cre-WPRE-oriP+/-VSVG

= Activity assay
= add 0.5 ml condition culture medium to 24 well plate, 293T cells transfected with 2181
3 days after adding medium



Retrieved from "https://eln.excellgen.com/index.php?title=Cre_VLP_Activity_assay_20140509&oldid=12000"