1038 pLysN-TEV: Difference between revisions
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Latest revision as of 12:48, 3 October 2014
Cloning of 1038 pLysN-TEV
[edit]Purpose: Cloning of1038 pLysN-TEV==
Date: 2014-9----
Notebook:0
Page:
'Clone ID:1038
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone from 2315 pEC2-TEV using Xba1 + BamH1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Xba1 + BamH1to find positive clone
Xba1 cleavage affected by meth
DO PCR screening using lysN-TEV-2 and plysN-seq-F
digest with HindIII+BamH1
