2373 pEC2-Trp63: Difference between revisions
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Latest revision as of 13:36, 3 November 2014
Cloning of 2373 pEC2-Trp63
[edit]Purpose: Cloning of2373 pEC2-Trp63==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2373
Experimenters: shanyi
Results
[edit]CLONING
[edit]Gene synthesis fragment 1
PCR from 2372 pSpeedET-Trp63 (fragment 2)
Gene Synthesis Fragment 3
join 3 fragments by pcr
digest with BamH1 + HindIII, clone to pEC1454
Colony Screening
[edit]Date: 2014_9
Make miniprep, use BamH1 + HindIII to find positive clone
