2352 pFastbac-GP64-Cre-Flpe-WPRE: Difference between revisions
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Latest revision as of 18:12, 20 November 2014
Cloning of 2352 pFastbac-GP64-Cre-Flpe-WPRE
[edit]Purpose: Cloning of2352 pFastbac-GP64-Cre-Flpe-WPRE==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2352
Experimenters: shanyi
Results
[edit]CLONING
[edit]subclone from 1972 pCMV5-Tat-Cre-PDGFR to 2354 pFastbac-GP64-Flp-WPRE using Nhe1 + Kpn1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Xho1 + Kpn1 to find positive clone