2240 pEC2-T4-uvsX: Difference between revisions
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Latest revision as of 13:58, 26 December 2014
Cloning of 2240 pEc2-T4-uvsX
[edit]Purpose: Cloning of2240 pEc2-T4-uvsX-==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2240
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR from T4 phage Genomic DNA
Fragment 1
T4-uvsX-Nde1-F tcgaacatatgtctgatttaaaatctcgtttg
T4-uvsX-R1 gtgtttcgtatgtatggttaatagc
Fragment 2
Cloning Method
gene synthesis
Join two fragments
T4-uvsX-Nde1-F tcgaacatatgtctgatttaaaatctcgtttg
T4-uvsX-Xho1-R (gatatggaagaatttaatgaa ctcgag tagca) tgctactcgagttcattaaattcttccatatc
digest with Nde1 + Xho1
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1 to find positive clone
sequence 2240a-5 with M13F primer, the second fragment is right.
Cloning of 2240 pEc2-T4-uvsX
[edit]Purpose: Cloning of2240 pEc2-T4-uvsX-==
Date: 2014-9----
Notebook:0
Page:
Clone ID:2240
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis the 1st fragment,pcr the 2nd fragment from 2240a-5
jOIN two fragment by PCR
digest with Nde1 + XhoI
Colony Screening
[edit]Date: 2014_8
Make miniprep, use Nde1 + Xho1 to find positive clone
