2378 pT7A3-Cre: Difference between revisions
Jump to navigation
Jump to search
No edit summary |
(No difference)
|
Latest revision as of 14:01, 26 December 2014
Cloning of2378 pT7A3-Cre
[edit]Purpose: Cloning of2378 pT7A3-Cre-==
Date: 2014-11----
Notebook:0
Page:
Clone ID:2378
Experimenters: shanyi
Results
[edit]CLONING
[edit]PCR Cre from 1708 pT7A3-HTNCre
2378-Cre-Nco1-F tagcaaccatgggcagcagctccaatttactgaccgtacac
2378-Cre-TEV-Nde1-R (gcctgctggaagatggcgatGGTACCggggatgacgatgacaaggaaaacctttacttccagggcCAtatgaacac) gtgttcataTGgccctggaagtaaaggttttccttgtcatcgtcatccccGGTACCatcgccatcttccagcaggc
Digest with Nde1-Nco1
Colony Screening
[edit]Date: 2014_11
Make miniprep, use Nde1-Nco1 to find positive clone
