2391 pEC2-pncA: Difference between revisions
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Latest revision as of 13:56, 8 January 2015
Cloning of2391 pEC2-pncA
[edit]Purpose: Cloning of2391 pEC2-pncA==
Date: 2014-12----
Notebook:0
Page:
Clone ID:2391
Experimenters: shanyi
Results
[edit]CLONING
[edit]gene synthesis
join two fragments by pcr
Digest with Nco1 + EcoR1
Colony Screening
[edit]Date: 2014_12
Make miniprep, use Nco1 + EcoR1 to find positive clone
fixing 2391_pEC2-pncA
[edit]Purpose: Cloning of2391_pEC2-pncA==
Date: 2014-12_2015_1
Notebook:0
Page:
Clone ID:2391
Experimenters: shanyi
Results
[edit]CLONING
[edit]use 2391-1 as template
join two fragment by pcr
clone to 2015 using Nde1 + Xho1
Colony Screening
[edit]Date: 2015_1
Make miniprep, use Nde1 + Xho1 to find positive clone
2392digest.JPG)